FastQCFastQC Report
Fri 17 Dec 2021
L-DM10-Plus_S6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM10-Plus_S6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31390933
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1870090.5957420889656259No Hit
ATCACACTTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTC720950.22966822935782125No Hit
ATGGATTCTGACTTAGAGGCCTTCAGTCATAATCCAACAGATGGTAGCTT674280.21480087896718456No Hit
ATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAGTCATAATCCAA638560.2034217969883214No Hit
CAAGAGTTCATATCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCC585420.18649334188314823No Hit
ATAATCCAACAGATGGTAGCTTCACGGCATTGTCTTTTCAGACAACCGTA584720.18627034755545493No Hit
CTCCCGTGTATATTTAGCTTTAGAAGAAGTTTATCTCCCACTTAGGGCTG570380.18170214947099533No Hit
AGAATTGTAATCTAAAGAGTTAACCTAAAGCTAAGCTTCTCGAATAAGTT548360.17468738504841508No Hit
CTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAGTCATAATCCAACAGA545930.17391327616799412No Hit
CGGCTCTTCCTATCATTGTGAAGCAGAATTCACAACGTGTCGGATTGTTC512760.1633465306685851No Hit
ATTTAAGTTAGTACGAGAGGAACCCTTAAATCAGATAATTGGTAAATACG505940.16117392879020193No Hit
AGTAATTTAAGTTAGTACGAGAGGAACCCTTAAATCAGATAATTGGTAAA501280.1596894236944152No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA498130.15868594921979542No Hit
TTCACGGCATTGTCTTTTCAGACAACCGTATTTACCAATTATCTGATTTA493030.15706127626088717No Hit
TTTTCGTTTATCATCACACTTAGACATTGCTTTCCAGCATGGATTCTGAC472780.15061036892404567No Hit
CAACAGATGGTAGCTTCACGGCATTGTCTTTTCAGACAACCGTATTTACC462690.1473960649720096No Hit
AATTTAAGTTAGTACGAGAGGAACCCTTAAATCAGATAATTGGTAAATAC444440.14158228428572034No Hit
GCTCTTCCTATCATTGTGAAGCAGAATTCACAACGTGTCGGATTGTTCAC434880.13853681889608058No Hit
ATTGTCTTTTCAGACAACCGTATTTACCAATTATCTGATTTAAGGGTTCC415610.1323981036180097No Hit
GTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA413260.1316494798036108No Hit
CATCACACTTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTT405110.1290531887026104No Hit
AGTTAGTACGAGAGGAACCCTTAAATCAGATAATTGGTAAATACGGTTGT391820.12481948210969072No Hit
CTTTTCAGACAACCGTATTTACCAATTATCTGATTTAAGGGTTCCTCTCG380400.12118148893503738No Hit
ACTTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAGTCA378080.12044242202039679No Hit
CTTCAGTCATAATCCAACAGATGGTAGCTTCACGGCATTGTCTTTTCAGA376880.12006014603006543No Hit
AGTCATAATCCAACAGATGGTAGCTTCACGGCATTGTCTTTTCAGACAAC374090.11917135435254504No Hit
AGCATGGATTCTGACTTAGAGGCCTTCAGTCATAATCCAACAGATGGTAG372020.11851192826922348No Hit
CACACTTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAG366120.11663240465009433No Hit
CACTTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAGTC361940.11530080995044015No Hit
CTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTC359200.11442794643918358No Hit
TGTTCACCCGCTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGAC354940.11307086667350727No Hit
TTAGAGGCCTTCAGTCATAATCCAACAGATGGTAGCTTCACGGCATTGTC352530.11230312905959182No Hit
CTTAGAGGCCTTCAGTCATAATCCAACAGATGGTAGCTTCACGGCATTGT346140.11026750941107738No Hit
TTATCATCACACTTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGG337340.10746415214864752No Hit
TTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAGTCATA335120.10675694156653452No Hit
GTTTTACCCTACTGATGAAACGATGTTGCGACAGTAATTTAAGTTAGTAC330590.10531384970303367No Hit
TTGTTCACCCGCTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGA326020.10385801530652179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG426800.050.17211
GCAGTGG465150.046.5245442
CGACGCT117800.046.2021681
CAGTGGT508700.042.692863
GACGCTC148350.036.0033452
ACGCTCT158500.033.34443
AGTGGTA673150.032.3878174
GTGGTAT706400.030.7942125
TGGTATC729700.029.839566
GGTATCA802950.027.152847
CGCTCTT197400.026.0819874
GTATCAA854000.025.5541328
GTGCTCT147400.025.1226481
AAGCAGT97000.023.4180221
TATCAAC959200.022.8426289
CTTCCGA685850.020.673262
TTCCGAT699300.020.1955263
TCTTCCG713450.019.9177271
ATCAACG1096350.019.87374710
CGATCTC187350.019.4666216
Produced by FastQC (version 0.11.4)