FastQCFastQC Report
Fri 17 Dec 2021
L-DM1-Plus_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM1-Plus_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20110634
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA757870.37685037677081684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG607810.30223313695629883No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528170.2626321974732373No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT514940.25605358836524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC137400.037.1731151
CAGTGGT206800.029.0157783
AGCAGTG215500.027.765071
GTGTGCT185050.027.4003472
AGTGGTA234250.025.7439884
TGTGCTC196900.025.709673
GCAGTGG244650.024.7042752
GTGGTAT294550.020.519555
GTGCTCT254800.019.8627344
GGTATCA328200.018.8289997
TGGTATC333750.018.6644366
GTATCAA330600.018.437968
TATCAAC362350.016.9192039
TGCTCTT296300.016.1226185
TTCCGAT414850.015.1224033
CTTCCGA423700.014.9505322
CAACGCA417950.014.44310112
TCCGATC435650.014.3005414
ATCAACG432450.014.22586210
TCAACGC430850.014.12521511
Produced by FastQC (version 0.11.4)