FastQCFastQC Report
Fri 17 Dec 2021
L-DM1-Next_S8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM1-Next_S8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23674236
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA553600.039.7451631
GGTATCA288200.037.1094361
TATCAAC823000.026.6259162
AACGCAG827950.026.1169366
CAACGCA845500.025.605915
AGAGTAC863450.024.95478611
CAGAGTA867100.024.84339710
GCAGAGT875050.024.5532369
ACGCAGA882600.024.5216147
ATCAACG889300.024.3443493
CGCAGAG886650.024.2944898
TCAACGC897200.024.1092934
AGTACGG840350.022.18301413
GAGTACG885000.021.13860712
TACGGGG519350.020.9828215
GTACGGG892600.020.85726514
TATACAG79300.017.39772
TACGGGA279800.016.6469915
TATACCG39150.015.8228765
ACGGGGG233550.015.79308916
Produced by FastQC (version 0.11.4)