FastQCFastQC Report
Fri 17 Dec 2021
L-DM1-Next_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM1-Next_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23674236
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATGTACTCTGTACTTCGTCATAGCACAGAACCCCGAGCTAAGCTAGG238360.10068329132141793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA587150.040.1244621
GGTATCA291550.037.5399361
TATCAAC872600.027.0384832
AACGCAG885950.026.5086386
CAACGCA899550.026.0533895
CAGAGTA928650.025.2788510
AGAGTAC930850.025.21513411
ATCAACG939350.024.9717143
GCAGAGT942750.024.8448839
ACGCAGA948000.024.7997367
TCAACGC945800.024.6942044
CGCAGAG950100.024.69328
AGTACGG880350.022.75826813
GAGTACG930500.021.6216412
GTACGGG932950.021.43013814
TACGGGG542000.020.66833315
TATACAG92300.018.9222182
TACGGGA281200.018.4065315
ATTATAC98400.017.9981043
TACTTTT197750.017.5732415
Produced by FastQC (version 0.11.4)