FastQCFastQC Report
Fri 17 Dec 2021
L-C5-Next_S16_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-C5-Next_S16_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27398997
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTCAGCAACT652530.23815835302292268No Hit
GTATCAACGCAGAGTACGGGAATATCAACCCGTTGTCCATCGACTACGCC593250.21652252452890883No Hit
ATATTATATGTCCCAGTGAGATTGCACCCCGTGTGCTAGAGGCACTTGCC592940.21640938170108928No Hit
CTCTTACACGCGCTTCAACCTGGCCATGGGTAGATCACTCCGCTTCGGGT573250.20922298725022673No Hit
ATATAATATCGTCCCCGTAGATGCCTATGGTTCCGGCGTTACCAAAATGG570660.20827769717263736No Hit
ATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGCCAC558480.20383227896991996No Hit
GATCAGTACCGTGAGGGAATGGTGAAAAGTACCCCGGGAGGGGAGTGAAA500510.18267457016765978No Hit
CCCCTACCCATCCAGCAAAACTGAATGCCACGGTTTCGGCGGTGTACTTG482380.17605753962453444No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCGCGCCGAC465870.17003177160098232No Hit
TATCAACGCAGAGTACGGGAATATCAACCCGTTGTCCATCGACTACGCCT409690.14952737138516423No Hit
GAATACTACCTGTTGACCGATAGCGGATCAGTACCGTGAGGGAATGGTGA403230.1471696208441499No Hit
GGGTAGATCACTCCGCTTCGGGTCTAGGACACGCGACTCAATCGCCCTAT391840.1430125343639404No Hit
CATATAGACGAACCAGTGTGGATGCTGGACCGTAGAGGGTGAGAGTCCCG387490.14142488500582703No Hit
ACCCACAGCTCATCCCCTCCATTTTCAACTGAAGTGGGTTCGGTCCTCCA383670.14003067338559874No Hit
CTACAAGGTTACTGACTTCAGCCAAACTCCGAATGCCGGGAAGTGAGAGC375860.1371802040782734No Hit
ACCTTGTAGGGCCCATCGTCCATCCAGTAGCTCTACCTCCGGGAAGAAAC352210.12854850124623174No Hit
GGGTAGGGGAGCGTCGTTCACGAGGTGAAGCCAGCGGGTAACTTCTGGTG348890.1273367780579705No Hit
CCTAAATGCATTTCGGGGAGAACCAGCTATCACGGAGTTTGATTGGCCTT347320.12676376438159395No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGT344590.12576737754305387No Hit
GTACTGATCCGCTATCGGTCAACAGGTAGTATTCAGGCTTACCAGGTGGT341690.12470894463764495No Hit
GTTGGAGGGTTGCCCGAGTAGCACGGGGCCCGAGGAATCCCGTGTGAATC334300.1220117656131719No Hit
CCCTAAGCGTGTGCTAAGTGGGAAAGGATGTGGAGTTGCTGAGACAACCA330930.12078179358171397No Hit
CGCTTAGGGGCCTTAACCGGTGGTCTGGGCTGTTTCCCTCTCGACTATGA311260.11360269866813007No Hit
CTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTCAGCAA309730.1130442840663109No Hit
GGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTCTTCAAAAGGC296650.10827038668605278No Hit
ACCCAGGGCAGATTAGCTTGACCCTGGAACCCTTAGTCATCCGGCGGACG289300.10558780673613709No Hit
CTTCTAAGCCAACCTCCTGGTTGTCTCAGCAACTCCACATCCTTTCCCAC288610.10533597270002257No Hit
GACTTACCCAGGGCAGATTAGCTTGACCCTGGAACCCTTAGTCATCCGGC287820.10504764097751462No Hit
CTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTT283610.10351108838035203No Hit
GGTATCAACGCAGAGTACGGGAATATCAACCCGTTGTCCATCGACTACGC282350.10305121753179504No Hit
GTTACTGACTTCAGCCAAACTCCGAATGCCGGGAAGTGAGAGCGTGGCAG279380.10196723624591075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA285400.044.2369081
GTATCAA580050.044.225991
TATTATA134400.037.153022
ATATTAT135000.037.0551341
ATTATAT135700.036.5827753
TTATATG133600.036.41594
TATATGT139900.034.7476965
ATATGTC142750.033.8554736
TATGTCC143600.033.3604437
ATGTCCC155500.031.0318728
CTATTGC143450.030.38982267
AGATTGC159600.030.1921619
GTGAGAT161450.029.99945616
GATTGCA158950.029.98637420
TGTCCCA161750.029.7468689
ACTATTG147050.029.62214966
TGACTAT149650.029.41239764
ATATAAT167350.029.2431811
TATAATA167950.029.2308792
ATCAACG888800.028.8446463
Produced by FastQC (version 0.11.4)