FastQCFastQC Report
Fri 17 Dec 2021
L-C5-Next_S16_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-C5-Next_S16_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27398997
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTCAGCAACT688260.25119897637128835No Hit
GTATCAACGCAGAGTACGGGAATATCAACCCGTTGTCCATCGACTACGCC682030.24892517050897886No Hit
ATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGCCAC646040.23578965317599035No Hit
CCCCTACCCATCCAGCAAAACTGAATGCCACGGTTTCGGCGGTGTACTTG623730.2276470193416204No Hit
CTCTTACACGCGCTTCAACCTGGCCATGGGTAGATCACTCCGCTTCGGGT595220.21724152895085902No Hit
ATATTATATGTCCCAGTGAGATTGCACCCCGTGTGCTAGAGGCACTTGCC561000.2047520206670339No Hit
GATCAGTACCGTGAGGGAATGGTGAAAAGTACCCCGGGAGGGGAGTGAAA550730.20100370827443062No Hit
ATATAATATCGTCCCCGTAGATGCCTATGGTTCCGGCGTTACCAAAATGG548050.2000255702790872No Hit
TATCAACGCAGAGTACGGGAATATCAACCCGTTGTCCATCGACTACGCCT483250.1763750694961571No Hit
GAATACTACCTGTTGACCGATAGCGGATCAGTACCGTGAGGGAATGGTGA439570.1604328800795153No Hit
GGGTAGATCACTCCGCTTCGGGTCTAGGACACGCGACTCAATCGCCCTAT438590.16007520275285989No Hit
CTACAAGGTTACTGACTTCAGCCAAACTCCGAATGCCGGGAAGTGAGAGC435410.15891457632554942No Hit
CCTAAATGCATTTCGGGGAGAACCAGCTATCACGGAGTTTGATTGGCCTT426850.1557903743702735No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCGCGCCGAC425780.155399849125864No Hit
CATATAGACGAACCAGTGTGGATGCTGGACCGTAGAGGGTGAGAGTCCCG418330.1526807714895549No Hit
GATCTACCCATGGCCAGGTTGAAGCGCGTGTAAGAGCGCGTGGAGGACCG399120.1456695659333807No Hit
GTACTGATCCGCTATCGGTCAACAGGTAGTATTCAGGCTTACCAGGTGGT380920.13902698700977995No Hit
CCCTAAGCGTGTGCTAAGTGGGAAAGGATGTGGAGTTGCTGAGACAACCA366980.1339392095265385No Hit
TCCTAGACCCGAAGCGGAGTGATCTACCCATGGCCAGGTTGAAGCGCGTG362400.1322676154897203No Hit
GGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTCTTCAAAAGGC356920.1302675422753614No Hit
CTTCTAAGCCAACCTCCTGGTTGTCTCAGCAACTCCACATCCTTTCCCAC345440.12607760787739786No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGT342200.12489508283825133No Hit
GACTTACCCAGGGCAGATTAGCTTGACCCTGGAACCCTTAGTCATCCGGC341220.12453740551159592No Hit
GCATTTAGGTGCAGCGTCACGTGTTTCTTCCCGGAGGTAGAGCTACTGGA338410.12351182052394108No Hit
ACCCAGGGCAGATTAGCTTGACCCTGGAACCCTTAGTCATCCGGCGGACG336620.12285851193749903No Hit
ACCCACAGCTCATCCCCTCCATTTTCAACTGAAGTGGGTTCGGTCCTCCA335700.12252273322267963No Hit
GGGTAGGGGAGCGTCGTTCACGAGGTGAAGCCAGCGGGTAACTTCTGGTG330820.1207416461266812No Hit
CCCCTACCCACAGCTCATCCCCTCCATTTTCAACTGAAGTGGGTTCGGTC315870.11528524201086632No Hit
GACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCAGGGCAGATT314010.11460638504394886No Hit
GGTATCAACGCAGAGTACGGGAATATCAACCCGTTGTCCATCGACTACGC309730.1130442840663109No Hit
GTGTAAGAGCGCGTGGAGGACCGAACCCACTTCAGTTGAAAATGGAGGGG302710.11048214648149346No Hit
GTTACTGACTTCAGCCAAACTCCGAATGCCGGGAAGTGAGAGCGTGGCAG288560.10531772385682585No Hit
CTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTT288110.10515348426805551No Hit
CTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTCAGCAA286800.10467536457630182No Hit
TCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACCCCACAAG285310.10413154904904001No Hit
GTACCGTGAGGGAATGGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTA284710.10391256293067953No Hit
ATGTAGCGGGGCTCAAGTACACCGCCGAAACCGTGGCATTCAGTTTTGCT279950.1021752730583532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303850.045.523451
GTATCAA636900.045.0410161
TATTATA132800.036.7395862
ATATTAT134350.036.420591
ATTATAT133250.036.2738463
TTATATG132600.036.108464
TATATGT141900.033.7913365
ATATGTC143900.033.4432336
TATGTCC144500.033.0827567
AGATTGC155700.031.13091319
GATTGCA154700.030.9471720
ATGTCCC155800.030.8628858
TGTCCCA160300.029.8436159
GTGAGAT161750.029.7930416
ATCAACG993050.028.999493
CAACGCA1002050.028.889225
AACGCAG1004350.028.8369466
CTATTGC131150.028.71871867
ACGCAGA1022000.028.35987
CAGAGTA1026950.028.28098110
Produced by FastQC (version 0.11.4)