FastQCFastQC Report
Fri 17 Dec 2021
L-C4-Next_S15_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-C4-Next_S15_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25929298
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATATGTCCCAGTGAGATTGCACCCCGTGTGCTAGAGGCACTTGCC725190.2796797661085927No Hit
ATATAATATCGTCCCCGTAGATGCCTATGGTTCCGGCGTTACCAAAATGG595550.22968226906875766No Hit
ATTTTAAAGAGAATGAGTTATCTTCAGTCTCACCGTCCGCGTAAACGCGA543920.20977043034485546No Hit
GTATCAACGCAGAGTACGGGCGACCCCACGATGACCCACTTCGCTTGTAG413030.1592908531499773No Hit
CTATTTATCTGACCGCACCGATCATTCGCCTCCCGTAGCTTAGCGATAGC347590.1340529928731584No Hit
GATAAATAGAGAAGGTTTCTTACATGACAAATCCTTGTCATGGGATCCGG335530.1294018835373021No Hit
GTATAGACCTGAATGATCAATCGATCAACCAGCGTCTGGCTCAGCAGGGC322990.12456565542190923No Hit
TATCAACGCAGAGTACGGGCGACCCCACGATGACCCACTTCGCTTGTAGG300320.11582264973004669No Hit
CCTTAGATGCGTTAGCATTAATCAGGCAACGGCTCTCTAGATAGAGCCCT267260.10307259378946548No Hit
GGTTTGACCTGTGCGAGCTTTTAGTACCCTTGATAGGGAGAACGAGACCT265040.10221641943410886No Hit
ACCATGGCTATCGCTGTAGGTAGCCGGAATTCCATTCCTAGGAGGTTTGA263390.10158007362945191No Hit
CTACCATGGCTATCGCTGTAGGTAGCCGGAATTCCATTCCTAGGAGGTTT260270.10037680156246422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA381450.052.687771
GTATCAA958100.045.4896431
TTATATG179300.034.7473344
TATGTCC180100.033.8304187
ATTATAT197400.033.1748053
ATATTAT195550.032.9876821
ATGTCCC187950.032.5477188
TATTATA209900.032.149722
ATATGTC193650.031.6484246
TGTCCCA194800.031.331169
TATCAAC1482550.029.5581722
CAACGCA1512500.029.1789425
ATCAACG1507700.029.1300563
TATATGT214000.029.096885
AACGCAG1521950.029.0621036
TGTGCTA208700.028.7761232
ACGCAGA1546700.028.565887
AGAGTAC1578050.027.96051811
TCAACGC1572400.027.9292094
GCAGAGT1581700.027.8583289
Produced by FastQC (version 0.11.4)