FastQCFastQC Report
Fri 17 Dec 2021
L-C2-Plus_S4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-C2-Plus_S4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20947159
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1681330.8026529993876496No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT893330.42646833396357No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT744920.35561863066967697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG607830.2901730015034497No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGG251760.12018813625274913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGG236290.11280288653941091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGG219730.10489727986501653No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215210.10273946934760939No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC158850.038.4147071
AGCAGTG274450.033.963951
CAGTGGT292750.031.7737643
TGTGCTC210750.028.5051773
GTGTGCT214400.028.2875732
AGTGGTA358250.025.9575214
GTGGTAT376000.024.683945
GCAGTGG399200.023.2975442
TGGTATC410400.022.9373746
GGTATCA468300.020.2709247
GTGCTCT296050.020.2038824
GTATCAA472600.019.886548
TATCAAC478850.019.6056659
TTCCGAT625700.017.7140873
TCTTCCG634350.017.709511
TCCGATC637350.017.3252374
CTTCCGA653300.017.1946872
CCGATCT624400.016.9939825
ATCAACG609000.015.47383910
CGATCTA229250.015.4479176
Produced by FastQC (version 0.11.4)