FastQCFastQC Report
Fri 17 Dec 2021
L-C2-Plus_S4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-C2-Plus_S4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20947159
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1698430.8108163975840351No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1126120.5376003495271126No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT977510.46665516789174133No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289440.13817625578724063No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG325700.036.8698731
CAGTGGT342500.035.0916023
CGACGCT129150.031.2466561
AGTGGTA416900.028.930034
GACGCTC140750.028.2485072
GCAGTGG433450.027.970772
GTGGTAT445450.027.1860315
TGGTATC491800.024.9795846
ACGCTCT170500.023.0320173
GTGCTCT121900.022.2231661
GGTATCA554650.022.1906057
GTATCAA558650.021.9502288
TCTTCCG550050.021.4689881
TATCAAC581650.020.9860429
TTCCGAT563950.020.8526943
CTTCCGA579200.020.4669062
TCCGATC583800.019.957924
CCGATCT577900.019.538015
CGCTCTT188450.019.4453644
ATCAACG735900.016.62549610
Produced by FastQC (version 0.11.4)