FastQCFastQC Report
Fri 17 Dec 2021
L-C2-Next_S11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-C2-Next_S11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15220011
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222000.041.3129841
GTATCAA410500.040.36621
AGATACT36900.028.93128845
AACGCAG583900.028.2476256
ACGCAGA588450.028.0269037
AGAGTAC593250.027.74352311
TATCAAC598900.027.631812
ATCAACG598600.027.5692983
TCAACGC599200.027.5061474
CAGAGTA607750.027.10392410
CAACGCA607400.027.0985455
GCAGAGT609700.027.0038979
CGCAGAG613750.026.8542128
TATTATA27950.024.5552392
ATGTAGA46450.024.34002934
GTAGAAT46550.024.21294636
AGTACGG664050.023.96631413
ATTATAT28300.023.2564953
GAGTACG686550.023.22644212
GATACTA47650.022.84503446
Produced by FastQC (version 0.11.4)