FastQCFastQC Report
Fri 17 Dec 2021
L-C2-Next_S11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-C2-Next_S11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15220011
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233400.041.3282851
GTATCAA446450.041.2835541
AACGCAG646950.028.7482456
ACGCAGA655300.028.4160487
TATCAAC650350.028.4045852
ATCAACG657250.028.2286823
AGAGTAC661600.028.0981311
CAGAGTA667850.027.84574710
TCAACGC666350.027.8430824
CAACGCA674550.027.5771665
CGCAGAG674750.027.5760158
GCAGAGT675000.027.5450639
AACTTAG29600.026.1282987
ATTATAT27000.025.4071333
AGTACGG724500.024.89615613
TATTATA28600.024.4754222
GAGTACG752450.024.04516412
AAACTTA31950.023.880656
GTACGGG795600.022.60529114
TTATATG28800.022.1177444
Produced by FastQC (version 0.11.4)