FastQCFastQC Report
Fri 17 Dec 2021
L-C1-Plus_S3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-C1-Plus_S3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27352756
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1719530.6286496322344995No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT939860.3436070573656271No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG934820.3417644642463085No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT570740.20865904700791393No Hit
TGCACTCCCACCTCCTTTATCACTTAGTGACAATTTTGGGGCCTTAGCTG324920.1187887611763875No Hit
AAAATTGTCACTAAGTGATAAAGGAGGTGGGAGTGCATTGACAACCAGGA305140.11155731437080783No Hit
AGAACGCTCCCCTACCGATATTTTCATATCCCACAGCTTCGGCATGTCAC281710.10299144992921372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCT234800.035.6136442
TGTGCTC239150.034.6925773
CGTGTGC248550.034.037131
CAGTGGT237250.033.4506033
AGTGGTA337400.023.8064464
GTGCTCT366100.022.6189254
GTGGTAT379750.021.1776335
TGGTATC389500.020.8792536
CGACGCT38650.019.396071
GGTATCA430200.018.9596867
TGCTCTT400250.018.4229165
GTATCAA441200.018.3599558
TCTTCCG796450.018.1960241
CGCTCTT98250.018.0412861
TTCCGAT797450.017.9020353
TATCAAC450800.017.8533259
TCCGATC807850.017.5591244
AGCAGTG475150.017.4951041
CCGATCT787300.017.469385
CTTCCGA826650.017.4142992
Produced by FastQC (version 0.11.4)