FastQCFastQC Report
Fri 17 Dec 2021
L-C1-Next_S10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-C1-Next_S10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22315486
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATAAAGGAGGTGGGAGTGCATTGACAACCAGGAGGTTTGCCTAGAAG1144160.5127201800579203No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGCGCTCCTGCGCCGAA925300.41464478972136215No Hit
CTTTCAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGTTGTCAATGCACTC855820.38350946064988234No Hit
GCCTATAAGTCGCCGGCTCATTCTTCAACAGGCACGCGGTCAGACGTTTA845420.3788490199137944No Hit
CCTTTATCACTTAGTGACAATTTTGGGGCCTTAGCTGGTGGTCTGGGCTG820490.36767740572622976No Hit
GACTAACCCTCCGTGGACGAACCTGCCGGAGGAACCCTTGGGGTTTCGGG815610.36549058353468084No Hit
CTTATAGGCAGTGGCAGGTTAAAGCACGTTGTGCCGTAGCCAAAGCGAAA795780.3566043777850054No Hit
GTATCAACGCAGAGTACGGGATTGTTGACCCGTTGTCCATCGACTACGCC759600.34039142145503803No Hit
CCCTTGGGGTTTCGGGGCATTGGATTCTCACCAATGTTTGCGCTACTCAA722160.3236138348051214No Hit
GTCCACACCTGCTTGCCGCTAGTGCTTCACCCTACCGCAGAACGCTCCCC612480.2744641098114556No Hit
GTTTCGGTGCGGGCTGCGAAAGCGGTACCAAATCGAGACAAACTCAGAAT588860.2638795319089174No Hit
GTAGTATTTAGCCTTACGAGATGGTCCTCGCAGATTCACACGGGATTTCT579620.2597389095626239No Hit
CCTTAGGTCCTGACTAACCCTCCGTGGACGAACCTGCCGGAGGAACCCTT548000.2455693772477104No Hit
GCCTAGAAGCAGCCACCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAG541920.24284481189430512No Hit
CTTCTGCTTCGTCCACACCTGCTTGCCGCTAGTGCTTCACCCTACCGCAG509340.2282450850499066No Hit
GGGCTAAGTGACATGCCGAAGCTGTGGGATATGAAAATATCGGTAGGGGA502960.22538608390603726No Hit
TATCAACGCAGAGTACGGGATTGTTGACCCGTTGTCCATCGACTACGCCG500110.22410894389662855No Hit
CACCGAAACAGTGCTTTACCCCCAAACTAAAATCTCAACCGCTGCGCCTC488830.21905415817517931No Hit
CCCCTAACCACACCTCATCCGCCGATTTTTCAACATCGGTCGGTGCGGAC482640.2162802996986039No Hit
GTGCGGACCTCCACTTGGTGTTACCCAAGCTTCATCCTGGACATGGTTAG479320.21479254361746816No Hit
GGTGTGGACGAAGCAGAAGTGAGAATGTCGGCTTGAGTAGCGCAAACATT460570.20639030671346348No Hit
GGTTAGGGGTGAAATGCCAATCGAACCCGGAGCTAGCTGGTTCTCCCCGA436780.19572954852966232No Hit
GGTCCGCACCGACCGATGTTGAAAAATCGGCGGATGAGGTGTGGTTAGGG423950.18998017789081537No Hit
GCCCAGACCACCAGCTAAGGCCCCAAAATTGTCACTAAGTGATAAAGGAG415570.18622493814385221No Hit
GCGTAATAGCTCACTGGTCAAGCGCTCCTGCGCCGAAAATGAACGGGGCT413740.18540487982202136No Hit
GGGTAACACCAAGTGGAGGTCCGCACCGACCGATGTTGAAAAATCGGCGG401420.17988405002696334No Hit
GTTGACCCGTTGTCCATCGACTACGCCGTTTGGCCTCGCCTTAGGTCCTG395000.17700712411103214No Hit
CTTCTAGGCAAACCTCCTGGTTGTCAATGCACTCCCACCTCCTTTATCAC393020.17611984789396926No Hit
AAATACTACTGTGTGACCGATAGTGAACCAGTACCGCGAGGGAAAGGTGA393000.1761108855079383No Hit
GTGCGTAATAGCTCACTGGTCAAGCGCTCCTGCGCCGAAAATGAACGGGG391130.17527290241404556No Hit
ACTTAGCCCCGTTCATTTTCGGCGCAGGAGCGCTTGACCAGTGAGCTATT390840.17514294781659695No Hit
CAGTAGTATTTAGCCTTACGAGATGGTCCTCGCAGATTCACACGGGATTT390540.17500851202613288No Hit
GTCCATCGACTACGCCGTTTGGCCTCGCCTTAGGTCCTGACTAACCCTCC381850.17111435529569016No Hit
GTGTTGAGGCGCAGCGGTTGAGATTTTAGTTTGGGGGTAAAGCACTGTTT376560.16874380419050697No Hit
TCCCACAGCTTCGGCATGTCACTTAGCCCCGTTCATTTTCGGCGCAGGAG374180.16767728025282533No Hit
GGTATCAACGCAGAGTACGGGATTGTTGACCCGTTGTCCATCGACTACGC370230.165907209011715No Hit
ATCTAACCATGTCCAGGATGAAGCTTGGGTAACACCAAGTGGAGGTCCGC369640.1656428186238023No Hit
GTGTTACCCAAGCTTCATCCTGGACATGGTTAGATCACCCGGGTTCGGGT369200.16544564613112167No Hit
CCTCAACACATTTCGGGGAGAACCAGCTAGCTCCGGGTTCGATTGGCATT357070.16000995900335757No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGTTGT356510.1597590121944913No Hit
GTCCAGGATGAAGCTTGGGTAACACCAAGTGGAGGTCCGCACCGACCGAT354730.1589613598377378No Hit
CTCTTTCAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGTTGTCAATGCAC349280.1565191096443071No Hit
GTACCAAATCGAGACAAACTCAGAATACCAAACTCACACTCAACCAGTGA345940.1550223911771404No Hit
CCCTACCGCAGAACGCTCCCCTACCGATATTTTCATATCCCACAGCTTCG342710.15357496583314384No Hit
GCGGTAGGGTGAAGCACTAGCGGCAAGCAGGTGTGGACGAAGCAGAAGTG337940.15143743676476507No Hit
GTAGGGGAGCGTTCTGCGGTAGGGTGAAGCACTAGCGGCAAGCAGGTGTG329160.14750294929718313No Hit
ATTTGGTACCGCTTTCGCAGCCCGCACCGAAACAGTGCTTTACCCCCAAA325330.14578665237225844No Hit
AAGCAGAAGTGAGAATGTCGGCTTGAGTAGCGCAAACATTGGTGAGAATC319490.14316963565122445No Hit
GCTTCGGCATGTCACTTAGCCCCGTTCATTTTCGGCGCAGGAGCGCTTGA313350.14041818313972637No Hit
CTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGCGCTCCTGCGCCGAAAA311830.13973704180137506No Hit
GTGGGGGATAAGCTTCATCGTCAAGAGGGAAACAGCCCAGACCACCAGCT309670.13876910411003374No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAA306820.137491964100625No Hit
GGTCTGGGCTGTTTCCCTCTTGACGATGAAGCTTATCCCCCACCGTCTCA303440.13597732086139644No Hit
GGTTATAGACCCGAACCCGGGTGATCTAACCATGTCCAGGATGAAGCTTG302460.13553816394588047No Hit
CTCCCCTACCGATATTTTCATATCCCACAGCTTCGGCATGTCACTTAGCC302060.1353589162252617No Hit
GAGTACGGGATTGTTGACCCGTTGTCCATCGACTACGCCGTTTGGCCTCG300900.13483909783546724No Hit
ATCCTGGACATGGTTAGATCACCCGGGTTCGGGTCTATAACCAGTGACGC296610.132916666031831No Hit
ACCAGGAGGTTTGCCTAGAAGCAGCCACCCTTGAAAGAGTGCGTAATAGC293100.1313437672834013No Hit
ATACTACTGTGTGACCGATAGTGAACCAGTACCGCGAGGGAAAGGTGAAA292100.13089564798185438No Hit
ACTCAAGCCGACATTCTCACTTCTGCTTCGTCCACACCTGCTTGCCGCTA284060.1272927687974172No Hit
ATCCCCCACCGTCTCACTGGTTGAGTGTGAGTTTGGTATTCTGAGTTTGT281470.12613213980641066No Hit
TTATAGACCCGAACCCGGGTGATCTAACCATGTCCAGGATGAAGCTTGGG278660.12487292456906382No Hit
GATCTAACCATGTCCAGGATGAAGCTTGGGTAACACCAAGTGGAGGTCCG270890.12139103759604428No Hit
CCCCAAGGGTTCCTCCGGCAGGTTCGTCCACGGAGGGTTAGTCAGGACCT261220.11705772395008561No Hit
CTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGTT260000.11651101840219837No Hit
GGGATAAGCTTCATCGTCAAGAGGGAAACAGCCCAGACCACCAGCTAAGG259410.11624662801428569No Hit
GTTGAAGAATGAGCCGGCGACTTATAGGCAGTGGCAGGTTAAAGCACGTT252190.11301120665711695No Hit
GCACTGTTTCGGTGCGGGCTGCGAAAGCGGTACCAAATCGAGACAAACTC250610.11230317816067281No Hit
TCTCACTTCTGCTTCGTCCACACCTGCTTGCCGCTAGTGCTTCACCCTAC249330.11172958545469278No Hit
GTATTTAGCCTTACGAGATGGTCCTCGCAGATTCACACGGGATTTCTCGT248250.1112456166090221No Hit
CGCCTTAGGTCCTGACTAACCCTCCGTGGACGAACCTGCCGGAGGAACCC247190.11077061014938235No Hit
CTCCTGCGCCGAAAATGAACGGGGCTAAGTGACATGCCGAAGCTGTGGGA244060.10936799673554051No Hit
GCTGTGGGATATGAAAATATCGGTAGGGGAGCGTTCTGCGGTAGGGTGAA242430.10863756227401905No Hit
GGAGGTGGGAGTGCATTGACAACCAGGAGGTTTGCCTAGAAGCAGCCACC241820.10836420950007541No Hit
GTACGGGATTGTTGACCCGTTGTCCATCGACTACGCCGTTTGGCCTCGCC236580.10601606435996958No Hit
AACCACACCTCATCCGCCGATTTTTCAACATCGGTCGGTGCGGACCTCCA236530.10599365839489222No Hit
CACCAATGTTTGCGCTACTCAAGCCGACATTCTCACTTCTGCTTCGTCCA235100.10535284779368015No Hit
GCTTATCCCCCACCGTCTCACTGGTTGAGTGTGAGTTTGGTATTCTGAGT234780.10520944961718512No Hit
ACATAGGGCTTACGGTGGATACCTAGGCACACAGAGGCGAAGAAGGACGT234460.10506605144069012No Hit
TGCCGAAGCTGTGGGATATGAAAATATCGGTAGGGGAGCGTTCTGCGGTA233220.10451038350677193No Hit
ATTCAGACTCGCTTTCGCTTTGGCTACGGCACAACGTGCTTTAACCTGCC232600.10423254953981285No Hit
GGTCCTGACTAACCCTCCGTGGACGAACCTGCCGGAGGAACCCTTGGGGT231620.10379339262429686No Hit
TTCTTCAACAGGCACGCGGTCAGACGTTTAATCGTCCTCCCACTGCTTGC230740.10339904763893558No Hit
GTTGTCCATCGACTACGCCGTTTGGCCTCGCCTTAGGTCCTGACTAACCC230730.10339456644592011No Hit
GTTATAGACCCGAACCCGGGTGATCTAACCATGTCCAGGATGAAGCTTGG229370.10278512419581631No Hit
CACTCAACCAGTGAGACGGTGGGGGATAAGCTTCATCGTCAAGAGGGAAA227960.10215327598063514No Hit
CACTGGTTGAGTGTGAGTTTGGTATTCTGAGTTTGTCTCGATTTGGTACC225250.100938872673443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA274150.050.498221
GTATCAA629550.042.91811
TCTACTT80350.033.817922
GTGCGCT86450.031.1674869
CTCTACT89600.030.6908591
TATCAAC883800.030.4521142
TTGTGCG88550.030.0322887
GCGCTAT90300.029.6871611
AACGCAG921350.029.2392586
ATCAACG922400.029.1366833
CAACGCA930100.028.9355755
ACGCAGA937600.028.7329447
CAGAGTA945050.028.54921210
GCAGAGT945250.028.5123429
TCAACGC942300.028.5002354
AGAGTAC949200.028.43024411
CGCAGAG972100.027.7058838
TACTTGT99550.027.6697924
ACGTTTA241700.027.55908444
TGTGCGC101800.027.0513178
Produced by FastQC (version 0.11.4)