FastQCFastQC Report
Fri 17 Dec 2021
L-C1-Next_S10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-C1-Next_S10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22315486
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATAGGCAGTGGCAGGTTAAAGCACGTTGTGCCGTAGCCAAAGCGAAA1169730.5241785905984749No Hit
GTGATAAAGGAGGTGGGAGTGCATTGACAACCAGGAGGTTTGCCTAGAAG1158280.5190476245957628No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGCGCTCCTGCGCCGAA989440.44338716172258136No Hit
CCTTTATCACTTAGTGACAATTTTGGGGCCTTAGCTGGTGGTCTGGGCTG923780.4139636483830108No Hit
CTTTCAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGTTGTCAATGCACTC923200.4137037391881136No Hit
GCCTATAAGTCGCCGGCTCATTCTTCAACAGGCACGCGGTCAGACGTTTA898110.4024604259123014No Hit
GACTAACCCTCCGTGGACGAACCTGCCGGAGGAACCCTTGGGGTTTCGGG891020.3992832600643338No Hit
GTATCAACGCAGAGTACGGGATTGTTGACCCGTTGTCCATCGACTACGCC790810.3543772248563173No Hit
CCCTTGGGGTTTCGGGGCATTGGATTCTCACCAATGTTTGCGCTACTCAA727620.3260605661915676No Hit
CCTTAGGTCCTGACTAACCCTCCGTGGACGAACCTGCCGGAGGAACCCTT599420.26861167173325284No Hit
GTTTCGGTGCGGGCTGCGAAAGCGGTACCAAATCGAGACAAACTCAGAAT580520.2601422169340161No Hit
GTCCACACCTGCTTGCCGCTAGTGCTTCACCCTACCGCAGAACGCTCCCC575910.25807638695388485No Hit
GCCTAGAAGCAGCCACCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAG560130.2510050643754745No Hit
TATCAACGCAGAGTACGGGATTGTTGACCCGTTGTCCATCGACTACGCCG554000.24825809305699192No Hit
GTAGTATTTAGCCTTACGAGATGGTCCTCGCAGATTCACACGGGATTTCT548380.2457396625822982No Hit
GGTCCGCACCGACCGATGTTGAAAAATCGGCGGATGAGGTGTGGTTAGGG514140.2303960576973318No Hit
AAATACTACTGTGTGACCGATAGTGAACCAGTACCGCGAGGGAAAGGTGA507560.22744743269315307No Hit
CTTCTGCTTCGTCCACACCTGCTTGCCGCTAGTGCTTCACCCTACCGCAG496750.22260326304343092No Hit
CACCGAAACAGTGCTTTACCCCCAAACTAAAATCTCAACCGCTGCGCCTC466920.20923586427828636No Hit
GGTTAGGGGTGAAATGCCAATCGAACCCGGAGCTAGCTGGTTCTCCCCGA464910.20833514448217708No Hit
CCCCTAACCACACCTCATCCGCCGATTTTTCAACATCGGTCGGTGCGGAC458780.2055881731636945No Hit
GGGCTAAGTGACATGCCGAAGCTGTGGGATATGAAAATATCGGTAGGGGA453830.20336998262103725No Hit
CAGTAGTATTTAGCCTTACGAGATGGTCCTCGCAGATTCACACGGGATTT434140.19454651357357847No Hit
CTTCTAGGCAAACCTCCTGGTTGTCAATGCACTCCCACCTCCTTTATCAC427320.1914903399370285No Hit
GGGTAACACCAAGTGGAGGTCCGCACCGACCGATGTTGAAAAATCGGCGG426060.19092570961707936No Hit
ATCTAACCATGTCCAGGATGAAGCTTGGGTAACACCAAGTGGAGGTCCGC415750.18630559961813065No Hit
ACTTAGCCCCGTTCATTTTCGGCGCAGGAGCGCTTGACCAGTGAGCTATT408070.18286404338225035No Hit
GGTGTGGACGAAGCAGAAGTGAGAATGTCGGCTTGAGTAGCGCAAACATT405130.1815465726357024No Hit
GCGTAATAGCTCACTGGTCAAGCGCTCCTGCGCCGAAAATGAACGGGGCT401800.18005433536155116No Hit
GTCCAGGATGAAGCTTGGGTAACACCAAGTGGAGGTCCGCACCGACCGAT395150.17707434200626418No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGTTGT393460.1763170203866499No Hit
GTACCAAATCGAGACAAACTCAGAATACCAAACTCACACTCAACCAGTGA392960.17609296073587644No Hit
GCCCAGACCACCAGCTAAGGCCCCAAAATTGTCACTAAGTGATAAAGGAG391840.17559106711814387No Hit
TCCCACAGCTTCGGCATGTCACTTAGCCCCGTTCATTTTCGGCGCAGGAG389730.17464553539187988No Hit
GTCCATCGACTACGCCGTTTGGCCTCGCCTTAGGTCCTGACTAACCCTCC387120.17347594401484243No Hit
CCTCAACACATTTCGGGGAGAACCAGCTAGCTCCGGGTTCGATTGGCATT382560.17143251999978848No Hit
GGTATCAACGCAGAGTACGGGATTGTTGACCCGTTGTCCATCGACTACGC375940.1684659702235479No Hit
ATACTACTGTGTGACCGATAGTGAACCAGTACCGCGAGGGAAAGGTGAAA373390.16732326600460326No Hit
CCCTACCGCAGAACGCTCCCCTACCGATATTTTCATATCCCACAGCTTCG372510.16692892101924198No Hit
AAGCAGAAGTGAGAATGTCGGCTTGAGTAGCGCAAACATTGGTGAGAATC360350.16147979031243148No Hit
GTGCGTAATAGCTCACTGGTCAAGCGCTCCTGCGCCGAAAATGAACGGGG356840.15990689156400179No Hit
GTTGACCCGTTGTCCATCGACTACGCCGTTTGGCCTCGCCTTAGGTCCTG347210.15559150269010497No Hit
GTGCGGACCTCCACTTGGTGTTACCCAAGCTTCATCCTGGACATGGTTAG344230.15425610717149518No Hit
CTCTTTCAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGTTGTCAATGCAC342710.15357496583314384No Hit
CTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGCGCTCCTGCGCCGAAAA335290.15024992061566572No Hit
ACCAGGAGGTTTGCCTAGAAGCAGCCACCCTTGAAAGAGTGCGTAATAGC331690.14863669113009684No Hit
GGTTATAGACCCGAACCCGGGTGATCTAACCATGTCCAGGATGAAGCTTG330380.14804965484507038No Hit
GAGTACGGGATTGTTGACCCGTTGTCCATCGACTACGCCGTTTGGCCTCG329910.1478390387733433No Hit
CTCCCCTACCGATATTTTCATATCCCACAGCTTCGGCATGTCACTTAGCC324590.14545504408911372No Hit
TTATAGACCCGAACCCGGGTGATCTAACCATGTCCAGGATGAAGCTTGGG323000.14474253439965412No Hit
GTAGGGGAGCGTTCTGCGGTAGGGTGAAGCACTAGCGGCAAGCAGGTGTG311220.13946368902743145No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAA307320.13771602375139846No Hit
GTTGAAGAATGAGCCGGCGACTTATAGGCAGTGGCAGGTTAAAGCACGTT307260.13768913659330564No Hit
GTGTTGAGGCGCAGCGGTTGAGATTTTAGTTTGGGGGTAAAGCACTGTTT306890.1375233324517333No Hit
ATTTGGTACCGCTTTCGCAGCCCGCACCGAAACAGTGCTTTACCCCCAAA303600.13604901994964394No Hit
CCCCAAGGGTTCCTCCGGCAGGTTCGTCCACGGAGGGTTAGTCAGGACCT300820.1348032482913435No Hit
GCTTCGGCATGTCACTTAGCCCCGTTCATTTTCGGCGCAGGAGCGCTTGA299470.13419828723425517No Hit
GATCTAACCATGTCCAGGATGAAGCTTGGGTAACACCAAGTGGAGGTCCG297970.13352610828193479No Hit
ACTCAAGCCGACATTCTCACTTCTGCTTCGTCCACACCTGCTTGCCGCTA295450.13239684764203657No Hit
ATCCCCCACCGTCTCACTGGTTGAGTGTGAGTTTGGTATTCTGAGTTTGT288800.12941685428674957No Hit
CTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGTT288550.12930482446136285No Hit
GCGGTAGGGTGAAGCACTAGCGGCAAGCAGGTGTGGACGAAGCAGAAGTG277420.12431725663514565No Hit
GGTCTGGGCTGTTTCCCTCTTGACGATGAAGCTTATCCCCCACCGTCTCA273660.12263232806132925No Hit
CACCAATGTTTGCGCTACTCAAGCCGACATTCTCACTTCTGCTTCGTCCA268640.12038276916756373No Hit
CACTCAACCAGTGAGACGGTGGGGGATAAGCTTCATCGTCAAGAGGGAAA263440.11805254879951976No Hit
ACATAGGGCTTACGGTGGATACCTAGGCACACAGAGGCGAAGAAGGACGT262380.11757754233988002No Hit
CGCCTTAGGTCCTGACTAACCCTCCGTGGACGAACCTGCCGGAGGAACCC259110.11611219222382162No Hit
GTTATAGACCCGAACCCGGGTGATCTAACCATGTCCAGGATGAAGCTTGG256330.11486642056552118No Hit
GTACGGGATTGTTGACCCGTTGTCCATCGACTACGCCGTTTGGCCTCGCC254200.11391192645322624No Hit
GTGTTACCCAAGCTTCATCCTGGACATGGTTAGATCACCCGGGTTCGGGT252790.1132800782380451No Hit
TCTCACTTCTGCTTCGTCCACACCTGCTTGCCGCTAGTGCTTCACCCTAC249210.11167581113850714No Hit
ATAGTGAACCAGTACCGCGAGGGAAAGGTGAAAAGAACCCCGGAAGGGGA248840.11151000699693478No Hit
GTCCAAGGATACTAGCTGAATCCCGAGTAGCACGGGGCACGAGAAATCCC248540.11137557120647071No Hit
TGCCGAAGCTGTGGGATATGAAAATATCGGTAGGGGAGCGTTCTGCGGTA245320.10993262705548963No Hit
GGGATAAGCTTCATCGTCAAGAGGGAAACAGCCCAGACCACCAGCTAAGG244530.10957861280726756No Hit
CTCCTGCGCCGAAAATGAACGGGGCTAAGTGACATGCCGAAGCTGTGGGA243300.10902742606636484No Hit
GCACTGTTTCGGTGCGGGCTGCGAAAGCGGTACCAAATCGAGACAAACTC240330.10769651174077051No Hit
CAACCAGTGAGACGGTGGGGGATAAGCTTCATCGTCAAGAGGGAAACAGC240070.1075800007223683No Hit
GTATTTAGCCTTACGAGATGGTCCTCGCAGATTCACACGGGATTTCTCGT238180.10673305524244464No Hit
CACTTAGCCCCGTTCATTTTCGGCGCAGGAGCGCTTGACCAGTGAGCTAT237440.10640144695929993No Hit
GGTCAAGCGCTCCTGCGCCGAAAATGAACGGGGCTAAGTGACATGCCGAA236220.10585474141141268No Hit
CACTGGTTGAGTGTGAGTTTGGTATTCTGAGTTTGTCTCGATTTGGTACC236140.10581889186728893No Hit
GTAAAGCACTGTTTCGGTGCGGGCTGCGAAAGCGGTACCAAATCGAGACA235870.10569789965587126No Hit
CCTCCTGGTTGTCAATGCACTCCCACCTCCTTTATCACTTAGTGACAATT231090.103555889394477No Hit
AGTCTGAATAGGGCGCAGCGTCACTGGTTATAGACCCGAACCCGGGTGAT229290.10274927465169256No Hit
GGTCCTGACTAACCCTCCGTGGACGAACCTGCCGGAGGAACCCTTGGGGT229060.10264620721233675No Hit
TTCTTCAACAGGCACGCGGTCAGACGTTTAATCGTCCTCCCACTGCTTGC227410.10190681036478436No Hit
GCCTAAACCAGTTGATTTATTAACTGGGGTAGTGGGACAGCGACATGGAA225870.1012167066404021No Hit
GCGCAGGAGCGCTTGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGC224080.10041457309063312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271250.050.6961751
GTATCAA645800.044.055691
TCTACTT76650.034.3374142
GTGCGCT83900.031.3250839
CATTCTT236450.030.85942319
TTGTGCG83750.030.6708287
ACGTTTA228050.030.61943244
GCGCTAT85950.030.33371411
CTCTACT88500.030.253881
TATCAAC940600.030.2440992
ATTCTTC241700.030.05840320
AGACGTT237300.029.9122942
GACGTTT233400.029.84245743
CGTTTAA235800.029.68728645
GTCAGAC246550.029.24360839
CAGACGT245450.029.21800641
ATCAACG980650.029.1873073
AACGCAG986650.029.126556
GCACGCG249150.029.02070432
GGCACGC249900.028.91934631
Produced by FastQC (version 0.11.4)