FastQCFastQC Report
Tue 7 Sep 2021
L-CSF8-DS_S1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-CSF8-DS_S1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12569552
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG1434051.140891894953774No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC1103680.8780583428908205No Hit
TTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC661100.5259535105149332No Hit
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG618110.4917518142253598No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA531060.42249715821216216No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT489450.3893933530805235No Hit
CGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA391990.3118567789846448No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC365940.29113209444537086No Hit
TCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT325260.2587681724853837No Hit
TCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGT304980.24263394590356124No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG288690.22967405680011507No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG277860.22105799793023648No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA274620.2184803404289986No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC258500.20565569878703713No Hit
CTCTTTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC256670.20419979964281942No Hit
GCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGG255800.20350765086933884No Hit
ATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA208800.1661157056353321No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT205630.16359373826529378No Hit
TCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCC202780.16132635435216783No Hit
CCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT172750.1374352880675461No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAAT171500.13644082143898206No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA170240.13543839907738955No Hit
TTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGAC160880.12799183296270225No Hit
GCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCAT160500.12768951510761878No Hit
CTTTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC155580.12377529445759085No Hit
GCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTC153410.12204890039040374No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC151180.12027477192504553No Hit
TTTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA149330.1188029613147708No Hit
TCCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGAC148170.11788009628346341No Hit
GGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTA147850.11762551282655101No Hit
TGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA144340.11483305053354327No Hit
CCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAA142610.11345670871961068No Hit
TTTGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC142250.11317030233058424No Hit
TGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGC139850.11126092640374136No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG137490.10938337340901251No Hit
CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCAT126290.10047295241707899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)