FastQCFastQC Report
Wed 5 Aug 2020
SRR942880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12189696
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTCG3764083.0879195018481185No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTA2450692.0104603100848455No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATTCG2262051.8557066558509745No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTC1190120.9763327977990591No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTAT858920.704627908686156No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA779020.6390807449176747No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGT739970.6070454915364583No Hit
TTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTCGTAT739050.6062907557333669No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATTCG725990.5955767887894825No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT686900.5635087208081317No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA635110.5210220172841061No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA619780.508445821782594No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCAA619570.5082735451318885No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCG586310.48098820512012774No Hit
TTGTATGGCTTCTAAACCATTTAAAGTTTACTGTAGGCACCATCAATTCG518290.4251869775915658No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC508780.41738530640961025No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATTC507610.4164254793556788No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTA480240.3939720892137097No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATTC437700.3590737619707661No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT432100.35447971795194894No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGTA418380.34322431010584675No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT408380.3350206600722446No Hit
AAATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCA403150.3307301511046707No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCTGTAGGCACCATCAATTCGTAT378520.3105245610719086No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATTCG352750.2893837549353159No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATTCGT336080.27570827032930106No Hit
TTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTCGTATG323370.26528143113659275No Hit
TCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATTCGTA318470.2612616426201277No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATT316390.25955528341313844No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTC299460.24566650390625No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATTC297240.24384529359879034No Hit
TTGAATTATTTAGTGAGGTCTCCGGACGTGCTGTAGGCACCATCAATTCG290820.23857855027721772No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATT286600.23511660996303765No Hit
ATGTATGTAAGCGTATTACCGGTGGCTGTAGGCACCATCAATTCGTATGC280760.23032567834341397No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAAT267870.21975117345010084No Hit
TGTATGGCTTCTAAACCATTTAAAGTTTACTGTAGGCACCATCAATTCGT261290.21435317172799057No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAAT258420.21199872416834678No Hit
AGTTACATATAAAAGCATTATTTAATACAATGCTGTAGGCACCATCAATT254830.2090536138062836No Hit
TGCACGCGCGCTACAATGAAAGTATCAACGCTGTAGGCACCATCAATTCG245970.20178517987651212No Hit
CGCTAGTTACATATAAAAGCATTATTTACTGTAGGCACCATCAATTCGTA232690.19089073263188844No Hit
ACGCGCGCTACAATGAAAGTATCAACGCTGTAGGCACCATCAATTCGTAT220780.18112018544186828No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATT219360.17995526713709678No Hit
AGGGAATACGGTTCCAATTCCGTAACCTGTTGAGCTGTAGGCACCATCAA213730.17533661216817875No Hit
TACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAA210870.17299036825856856No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATTC209620.17196491200436828No Hit
CGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTC197630.1621287356140793No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGT193070.15838787119875672No Hit
TTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAAT191830.15737061859459006No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA187790.15405634398101478Illumina Single End Adapter 1 (95% over 22bp)
TACACACAGGGACAGCAAAAGCTCGGCCTATCGCTGTAGGCACCATCAAT187650.15394149288054437No Hit
AGTTCGTGGAGTGATTTGTCTGGTTCTGTAGGCACCATCAATTCGTATGC185810.15243202127436156No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCTGTAGGCACCATCAATTCGTATG182400.14963457661290322No Hit
TTGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATTCGT177030.14522921654485887No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCA167290.13723886141213038No Hit
GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTCTGTAGGCACCATCAATT165340.13563914965557797No Hit
TATGCACTTGTATGATTAACAATGCGCTGTAGGCACCATCAATTCGTATG161450.13244792979250672No Hit
TGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTAT161070.13213619109122984No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATT161040.13211158014112903No Hit
CGCTAGTTACATATAAAAGCATTATTTCTGTAGGCACCATCAATTCGTAT155940.12792771862399194No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATT146220.11995377079133064No Hit
AGCTATAAAATAAATTTTTAAAAATATTCTGTAGGCACCATCAATTCGTA146020.1197896977906586No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCTGTAGGCACCATCAATTCGTAT144730.11873142693632392No Hit
ATTTAGAAATTATAAATTTACTTAATTATTCTGTAGGCACCATCAATTCG144130.11823920793430778No Hit
TTCGCAAAAGTTGACCGAACTTGATTATTTAGCTGTAGGCACCATCAATT144080.1181981896841398No Hit
ATCAGTTATGGTTCCTTAGATCGTTCTGTAGGCACCATCAATTCGTATGC142460.11686919837869623No Hit
TCGCAAAAGTTGACCGAACTTGATTATTTAGCTGTAGGCACCATCAATTC141830.1163523684265793No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATTC132810.10895267609627017No Hit
TATCCACTGAAGCTTATCCTTTGCTTGCTGTAGGCACCATCAATTCGTAT132710.10887063959593413No Hit
ATTGAATTATTTAGTGAGGTCTCCGGACGTGCTGTAGGCACCATCAATTC130690.10721350228914651No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGACTGTAGGCACCATCAATTCG130210.1068197270875336No Hit
ATTGAATTATTTAGTGAGGTCTCCGGACGTGACTGTAGGCACCATCAATT127360.10448168682795698No Hit
ATGGCAAATGTGAGATGCAGTGTATGGCTGTAGGCACCATCAATTCGTAT123380.10121663411458333No Hit
ATAAGCATATGGTATATTATTGGATAAACTGTAGGCACCATCAATTCGTA122120.10018297421034947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)