FastQCFastQC Report
Wed 5 Aug 2020
SRR942879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8192000
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGTAAGCGTATTACCGGTGGCTGTAGGCACCATCAATTCGTATGC1983372.4211059570312496No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATTCG1859722.270166015625No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTCG1858802.26904296875No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTA1254081.530859375No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTC663650.8101196289062499No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGT650340.7938720703125001No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT508130.62027587890625No Hit
TTATGTATGTAAGCGTATTACCGGTGGCTGTAGGCACCATCAATTCGTAT489480.597509765625No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATTC434980.5309814453125No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA434200.530029296875No Hit
TTGTATGTAAGCGTATTACCGGTGGCTGTAGGCACCATCAATTCGTATGC417810.5100219726562499No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCG405970.49556884765625003No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGTA376200.45922851562499994No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT363760.44404296875No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA363440.44365234375No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTAT296360.361767578125No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCATCAA260180.3176025390625No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA243870.29769287109375No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATT235100.2869873046875No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATT232600.283935546875No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATTC228330.27872314453124997No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT222810.27198486328125No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC222140.27116699218749996No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTA209620.2558837890625No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAAT207770.25362548828125003No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATT191660.23395996093750002No Hit
TTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTCGTAT182850.22320556640625No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGT172960.2111328125No Hit
TCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATTCGTA166470.20321044921874998No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATTCG145630.17777099609375No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCA145240.17729492187499998No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCAA144770.17672119140625No Hit
ATTTATCATAAAATATAACTTATAGTTTATTCCTGTAGGCACCATCAATT138260.16877441406250002No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCTGTAGGCACCATCAATTCGTAT130440.159228515625No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTC129860.15852050781250002No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCCTGTAGGCACCATCAAT127260.15534667968750002No Hit
ACGCGAAGGTCGCGGGTTCGTTCCCCTCATGGGCCACCACTGTAGGCACC123310.15052490234375No Hit
AAATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCA115920.14150390625No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATTCGT115180.14060058593750002No Hit
TTAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTC114500.1397705078125No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATCTGTAGGCACCATCAAT112130.13687744140625No Hit
AGCTATAAAATAAATTTTTAAAAATATTCTGTAGGCACCATCAATTCGTA110050.13433837890625No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATTC109960.13422851562500002No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATT108800.1328125No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATT106270.12972412109375No Hit
ATTTATCATAAAATATAACTTATAGTTTATTCTGTAGGCACCATCAATTC104790.12791748046874998No Hit
CGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTC104720.12783203125No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAA101900.1243896484375No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATTC101250.12359619140625No Hit
ATCAGTTATGGTTCCTTAGATCGTTCTGTAGGCACCATCAATTCGTATGC100560.12275390624999999No Hit
TTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTCGTATG96670.11800537109374999No Hit
TTTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATTCGT96020.1172119140625No Hit
TTATAAGCATATGGTATATTATTGGATAAACTGTAGGCACCATCAATTCG94200.114990234375No Hit
TGCATTTTAACACAGAATAAATGTTATCTGTAGGCACCATCAATTCGTAT93220.11379394531249999No Hit
TGTATGTAAGCGTATTACCGGTGGCTGTAGGCACCATCAATTCGTATGCC89710.10950927734375No Hit
TGTTACGGTTGTTTCGCAAAAGTTGACCGAACTCTGTAGGCACCATCAAT87600.10693359375No Hit
TACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAA83360.1017578125No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATT82590.10081787109375001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)