FastQCFastQC Report
Wed 5 Aug 2020
SRR942877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20217856
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC15615257.723494518904478No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA4463112.2075090454695094No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC3408551.685910711798521No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCG3213631.5895008847624594No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC2913171.4408896769271677No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCA2781921.3759718142220423No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT2695811.3333807501646069No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCT2495361.2342357171799028No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT2114651.04593187329062No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT1556970.7700964929219003No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCTC1496710.7402911564905794No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAA1373910.6795527676129457No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCT1331820.6587345364414506No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA1322040.65389722827188No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA1059390.5239873110185372No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA988680.48901327618517015No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA830970.41100797235869124No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC813510.40237204182283226No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT811930.4015905544089343No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCATCAATAT686450.3395266046013979No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT670250.3315138855475081No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA645550.31929696205176256No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATATC518010.2562141109324352No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT459870.22745735255014182No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAT441030.21813885705784034No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCATCAA431760.2135538011547812No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA408440.2020194426154781No Hit
AAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC404080.19986293304294975No Hit
CTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA397560.19663806092990277No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATAT378030.18697828295938007No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA366610.18132981063867504Illumina PCR Primer Index 6 (96% over 29bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCTC361180.17864406591876011No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATAT357630.17688819229892627No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAT356990.1765716404350689No Hit
TGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCA341280.1688012814019449No Hit
CAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC337210.1667882093927269No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC330810.16362269075415317No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATA325490.16099135338583873No Hit
ACGCGAAGGTCGCGGGTTCGTTCCCCTCATGGGCCACCACTGTAGGCACC298810.14779509756128445No Hit
GCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATATCTCGTAT297930.14735983874848055No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATATC287330.14211694850334278No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATAT284980.1409546096282415No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCAA278480.13773962976094004No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATAT275250.13614203207303485No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATAT269090.13309522038340763No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGTAGGCACCATCAATA260020.1286090869378039No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATC255220.12623494795887358No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATATCT251980.12463240414809564No Hit
GTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA243900.12063593686689628No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATA241260.1193301604284846No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATATC239880.11864759547204214No Hit
TGTTACGGTTGTTTCGCAAAAGTTGACCGAACTCTGTAGGCACCATCAAT237940.11768804763472449No Hit
TTAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATAT235110.11628829486173015No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT231320.11441371429294976No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATA229290.11340965134977715No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCCTGTAGGCACCATCAAT214990.10633669564171393No Hit
AGCAGAGTTGCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT206190.10198410751367504No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAAT203180.10049532452897084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)