FastQCFastQC Report
Wed 5 Aug 2020
SRR942876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17235968
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC14914038.652853149878208No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA3378001.9598551122861216No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCT2870201.665238645140209No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC2841351.6485003917389496No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCG2822411.6375117428855752No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC2676711.5529792118435124No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT2346581.3614436972730513No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCA2085771.2101264054331036No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT1647100.9556179264199145No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCTC1361930.7901673987791112No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC1361780.7900803714650665No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCT1274000.7391519872861216No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT1131630.6565514626158507No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1125880.6532154155774715Illumina PCR Primer Index 6 (96% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA1057740.613681807717443No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAA1025280.5948490969581749No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA965120.5599453422053232No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA954570.5538244211175143No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA787660.4569862278695342No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT704990.4090225741890447No Hit
GCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATATCTCGTAT685430.3976742124376188No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC542250.31460374027150667No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT489720.2841267748930608No Hit
TGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCA481880.2795781472789924No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA459590.2666458884119534No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATATC438020.2541313606523289No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT405390.23520001893714357No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATATCTCGTATG395920.22970569451045628No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA391280.22701364959600762No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCATCAATAT366320.21253230453897337No Hit
AAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC341080.19788850849572245No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAT338290.19626980045449144No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATAT329870.1913846672261169No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCTC328850.19079288149061313No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCATCAA313280.18175944629277568No Hit
CTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA301550.17495391033448193No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAT295570.1714844214145675No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATAT288310.16727229941480515No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATAT283910.16471949820282794No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATC281980.16359974676211977No Hit
CAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC280620.16281069911478135No Hit
TGTTACGGTTGTTTCGCAAAAGTTGACCGAACTCTGTAGGCACCATCAAT280590.16279329365197243No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATA263770.153034630837096No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATAT243080.1410306633198669No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATAT232060.13463705664805134No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC230860.1339408381356939No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATATC229380.13308216863711977No Hit
ACGCGAAGGTCGCGGGTTCGTTCCCCTCATGGGCCACCACTGTAGGCACC222900.12932258867038973No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCAA220570.1279707643922291No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT218150.12656672372564165No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATA214600.12450707729325095No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGTAGGCACCATCAATA192260.11154580932153041No Hit
GTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA183690.10657364877911123No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATA183280.10633577412072243No Hit
AGCAGAGTTGCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT181490.10529724817312262No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA181260.10516380629158745No Hit
AGCAGAGTTGCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA173340.10056876411002852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)