FastQCFastQC Report
Wed 5 Aug 2020
SRR942874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13074432
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC9154337.001703783384242No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA3868562.9588742363721803No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT2469381.8887091997572054No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC2369721.8124840910870927No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT2361211.8059752041235901No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCA2305991.7637400997611217No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC2090871.599205227424029No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCT1671291.2782888006148183No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCG1506851.15251660645755No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA1366061.0448331522164787No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA1227050.93851113379151No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAA1204200.9210342751409775No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA1093500.8363652050046991No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT1062130.8123718108748434No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA885670.6774061007009712No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCT860340.6580324101268797No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC805780.616302107808584No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA769460.5885226983474311No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAT737560.5641239328790727No Hit
TGTTACGGTTGTTTCGCAAAAGTTGACCGAACTCTGTAGGCACCATCAAT623970.47724444167058266No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCTC608130.46512919261043234No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT565910.4328371588150063No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC562360.4301219356986215No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATAT465080.35571717379385964No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAATAT425220.32523018973214285No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA422520.3231650904605263Illumina PCR Primer Index 6 (96% over 29bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT406820.3111569206218672No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAT390890.2989728349193296No Hit
GCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATATCTCGTAT374810.28667402147947996No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATAT369010.2822378823034148No Hit
CTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA341300.2610438449639724No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAT330870.25306644296287595No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC314860.2408211691337719No Hit
AACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCAATATCTCG308120.23566606947055135No Hit
AGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGCTGTAGGCACCATC295060.22567710780858397No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCCTGTAGGCACCATCAA292430.22366554814771306No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGCTGTAGGCACCATCA278630.21311059631500626No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATATC276060.21114492774906013No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATATCT260290.1990832182996554No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGTAGGCACCATCAAT248680.19020329143170425No Hit
AAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC243750.1864325731320489No Hit
ATATGGGACTACCCCCTGAATTCTGTAGGCACCATCAATATCTCGTATGC241970.1850711373159461No Hit
AGGGAATACGGTTCCAATTCCGTAACCTGTTGAGCTGTAGGCACCATCAA240940.1842833401864035No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATA239770.18338846383536966No Hit
TTGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAATAT238290.18225648349389098No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATAT234330.17922767122885339No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCTC233010.17821806714050753No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATATCTC231040.17671130952380953No Hit
CTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT222140.16990413044329575No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT221150.16914692737703635No Hit
CAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC209210.16001459948699875No Hit
GTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA207470.1586837577341792No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATATCT199920.1529091282894737No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATAT198940.1521595737390351No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAAT189640.14504645402568922No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTCCTGTAGGCACCATCAAT187910.14372326078869047No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATC187210.14318786468123434No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA183490.140342616795896No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCCTGTAGGCACCATCAAT183050.1400060820997807No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATATCTCGTATG182670.13971543849859022No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGCTGTAGGCACCATCAATA174990.13384137834821427No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCA174970.13382608131657267No Hit
TTGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATATCT174180.13322184856672933No Hit
TGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCG169840.12990239270050125No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATA167710.12827325883067042No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCATCAATAT166800.12757724389097747No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA166770.12755429834351503No Hit
AACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAATATCTCGTA165130.1262999417489035No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGTAGGCACCATCAATA159520.12200912437343359No Hit
GATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCGT158070.1209000895794173No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATAT156190.11946216860510653No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCATCAA152380.11654808407738095No Hit
AACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAATATCTCGT149570.11439885113173559No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAAT148860.11385580650845864No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAAT148180.11333570743264411No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATATCT147190.11257850436638471No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATATC139490.10668914718436717No Hit
ACGGTTGTTTCGCAAAAGTTGACCGAACTCTGTAGGCACCATCAATATCT138200.10570248864348372No Hit
GTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT137630.105266523241698No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATA136850.10466993900767545No Hit
CTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT136740.10458580533364661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)