FastQCFastQC Report
Wed 5 Aug 2020
SRR942870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60620800
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCAC28153724.644234322212838No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCA22629403.732943148226351No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA18365323.029541015625No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA17141462.8276532147381754No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT10349821.7073050834037162No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCACTGTAGGCACC8345321.3766430004222971No Hit
TGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCA7639561.2602209142736487No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCACTGTAGGCACCA7246941.1954543654983107No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTC5537140.913405959670608No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCA5111730.8432303763724662No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT4742070.7822513064822635No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTA4100510.6764196447423987No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTCG3882440.6404468433277027No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATTC3567810.5885455157305743No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGTA3184020.5252355627111486No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGT3077240.5076211465371622No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATT2596330.4282902898015203No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA2406540.3969825538429054No Hit
AGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGCTGTAGGCACCATC2276250.3754899308488176No Hit
TAACCAAACGTAATGTTACGGTGCCCAAATTAACAACCTGTAGGCACCAT2150750.35478746568834457No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT2064920.34062895903716217No Hit
AACTCCATCTAAAACTAAATATAACCATGAGACCGCTGTAGGCACCATCA1822060.30056680215371623No Hit
TTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACC1752770.2891367319467905No Hit
AACTCCATCTAAAACTAAATATAACCATGAGACCGACTGTAGGCACCATC1745960.28801335515202703No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAAT1735410.28627302839949326No Hit
AGGGAATACGGTTCCAATTCCGTAACCTGTTGAGCTGTAGGCACCATCAA1613110.2660984348606419No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATT1548330.2554123337204392No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCAA1539640.25397883234797297No Hit
TGGGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCAC1507880.24873970650337837No Hit
AAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGCTGTAGGCACCAT1504960.24825802364864866No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTCCTGTAGGCACCATCA1460590.24093875369510134No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGT1441300.23775667757601351No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGCTGTAGGCACCATCA1416900.23373165646114863No Hit
TGGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCA1412360.23298273859797294No Hit
CGCTAGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCA1380770.22777165593327703No Hit
TGAACCAAACGTAATGTTACGGTGCCCAAATTAACAACCTGTAGGCACCA1377510.22723388671874997No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCATCAA1308050.21577577333192566No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAAT1301710.21472992768158786No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATCA1276500.2105712890625No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCACTGTAGGCACCATC1262340.20823545713682434No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTCTGTAGGCACCATC1261640.2081199852195946No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCACTGTAGGCACCAT1245460.20545093433277026No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATT1202950.19843848975929054No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTAT1200860.19809372360641894No Hit
TAACCAAACGTAATGTTACGGTGCCCAAATTAACAACTCCTGTAGGCACC1180790.1947829787795608No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA1178940.1944778029983108No Hit
TCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATTCGTA1145270.1889236037795608No Hit
TAACCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGCTGTAGGCACC1141950.18837593697212837No Hit
TTTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCAC1130490.18648549672719594No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATTCGT1126420.18581411000844594No Hit
AAATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCA1103430.18202168232685811No Hit
ACCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGCTGTAGGCACCAT1092990.18029950116131757No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATT1089840.17977987753378377No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAATTC1081970.17848164326435811No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC982660.1620994774070946No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT948360.15644135346283783No Hit
CTGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCA896710.14792117557010134No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGCTGTAGGCACCATCAATT873580.14410565350506757No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCG871850.14382027290962837No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATTC863870.14250389305320946No Hit
ACGCGAAGGTCGCGGGTTCGTTCCCCTCATGGGCCACCACTGTAGGCACC842890.1390430347339527No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATTC835280.13778769003378377No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATT833760.13753695101351351No Hit
ACCAAACGTAATGTTACGGTGCCCAAATTAACAACCTGTAGGCACCATCA832680.1373587943412162No Hit
GTGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCA828860.1367286475929054No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC789240.1301929370777027No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTCACTGTAGGCACCATC786460.12973434860641891No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGATTCGACTGTAGGCA768140.12671228357263511No Hit
TGGAACGTACCTTGAGCATATATGCTGTGACCCGACTGTAGGCACCATCA762270.12574396906672297No Hit
ATAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA759100.12522104624155406No Hit
CGCTAGTTACATATAAAAGCATTATTTAATACAATGCTGTAGGCACCATC746430.12313100453969594No Hit
ACCGAAAGGTTGGTGGTTCGTGTCCACCCGGGGGCGCCACTGTAGGCACC717650.11838345914273649No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATT705530.11638414537584459No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGT686190.11319382126266891No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTC613520.1012061866554054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)