FastQCFastQC Report
Wed 5 Aug 2020
SRR7262146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7262146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10911744
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG10446199.57334592893675Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1087240.9963943435623124Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGC943650.8648021801097974No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA856080.7845491976351352RNA PCR Primer, Index 1 (100% over 22bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA833220.7635992926520271RNA PCR Primer, Index 1 (100% over 22bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG778500.7134514885979729Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG667600.61181787256006No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCC625320.5730706292229729No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAAG622240.5702479823573574No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA615510.5640803156672297No Hit
TGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAAGG592140.5426630243524775Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGAA590430.5410959054757883RNA PCR Primer, Index 1 (100% over 23bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA506650.46431624495589335RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTGC487920.44715125281531537No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG426320.390698315503003Illumina Small RNA Adapter 2 (100% over 21bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG366080.33549174174174173No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG347640.31859251829954954No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA330860.3032145915446697No Hit
AGGGAATACGGTTCCAATTCCGTAACCTGTTGAGTGGAATTCTCGGGTGCC297490.27263286235454204No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA285070.2612506305133258RNA PCR Primer, Index 1 (100% over 23bp)
ATGGCAAATGTGAGATGCAGTGTATGGAGTGGAATTCTCGGGTGCCAAGGA224980.20618152332019518RNA PCR Primer, Index 1 (100% over 22bp)
ATATGGCAAATGTGAGATGCAGTGTATGGAGTGGAATTCTCGGGTGCCAAG195980.17960465348160662No Hit
AATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTG185300.1698170338307057No Hit
TGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGGAATTCTCGGGTGCCAA169960.15575878612987987No Hit
ACATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAG162330.14876632003096846No Hit
TATGGCAAATGTGAGATGCAGTGTATGGAGTGGAATTCTCGGGTGCCAAGG156970.14385418132976727Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC156670.143579248193506RNA PCR Primer, Index 7 (100% over 51bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCATGGAATTCTCGGGTG139430.12777975729635888No Hit
TATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAA135770.12442557303397148No Hit
TCGACATTTTGAACGCATATCGCAGTCCATGCTGGAATTCTCGGGTGCCAA135080.12379322682057056No Hit
ACGGCAATTTTTGGAAGCCCGAGAGGGCTAATGGAATTCTCGGGTGCCAAG129540.11871612823761261No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCAA121490.11133875574793543No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA121360.11121961805555555No Hit
TGGAACGTACCTTGAGCATATATGCTGTGTGGAATTCTCGGGTGCCAAGGA119130.10917594840934686RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)