FastQCFastQC Report
Wed 5 Aug 2020
SRR7262143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7262143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27951104
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG323335211.567886549311254Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG18132356.48716773405444Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA8871883.1740714069827076RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA6722632.4051393461954134No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA6586962.356601012968933RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG6409742.2931974350637456No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG5248721.8778220709994138No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCAA4203111.5037366681473476No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAA2899851.0374724375824298RNA PCR Primer, Index 1 (100% over 23bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA2423060.86689241326568RNA PCR Primer, Index 1 (100% over 23bp)
ATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAAC2224880.7959900260111372RNA PCR Primer, Index 1 (100% over 24bp)
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG1985090.7102009280205891Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA1938470.6935218015002198No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTGGAATTCTCGGGTGCCAAG1398360.5002879313818874No Hit
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGAA1336320.4780920281359906RNA PCR Primer, Index 1 (100% over 23bp)
ACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCAAGGAA1154090.412896034446439RNA PCR Primer, Index 1 (100% over 23bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA846450.3028324033283265RNA PCR Primer, Index 1 (100% over 22bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG735430.26311304197501467No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA716380.2562975687829719No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGCC704970.2522154402201788RNA PCR Primer, Index 8 (100% over 51bp)
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGC616200.22045640844812428No Hit
TCGGCTGAGTTGACCTCTGCGATTATTCCTGGAATTCTCGGGTGCCAAGGA514980.18424316978678193RNA PCR Primer, Index 1 (100% over 22bp)
TTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCAAGG486680.17411834609466587Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAAC478150.17106658828216587RNA PCR Primer, Index 1 (100% over 24bp)
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGCC440560.15761810338511137No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGTGCCAAGGA414460.1482803684605803No Hit
TACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCAAGGA395800.14160442464097303RNA PCR Primer, Index 1 (100% over 22bp)
TATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAA391690.14013399971607562RNA PCR Primer, Index 1 (100% over 23bp)
ACCTCGGCTGAGTTGACCTCTGCGATTATTCCTGGAATTCTCGGGTGCCAA377290.1349821459646102No Hit
CTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG369450.1321772478110346No Hit
TGCTGGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG369310.13212716034400646Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGCTGAGTTGACCTCTGCGATTATTCCTTGGAATTCTCGGGTGCCAAGG342580.12256403181784878Illumina Small RNA Adapter 2 (100% over 21bp)
TGGTTTATGTGCTCGTTCTGGGTAAATAGAGTGGAATTCTCGGGTGCCAAG333160.11919386082209847No Hit
TAGTTACGTAGCCAATTGTGGAACTTTCTTGCTGGAATTCTCGGGTGCCAA308120.11023535957649473No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGA307730.11009583020405919RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGAA298860.10692243140020515RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)