FastQCFastQC Report
Mon 22 Feb 2021
ZT8_II_PdfG_plus_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZT8_II_PdfG_plus_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24239872
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5105782.106356007160434No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3378541.3937944886837685No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2657921.0965074403033153No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000060.8251116177511169No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1663980.6864640209321238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1266790.5226058949486202No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1212170.5000727726615058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT864230.3565324107322019No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT691630.28532741427017433No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690730.28495612518085905No Hit
GAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660590.2725220661231214No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA658180.27152783645062156No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500590.20651511691150842No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344020.14192319167361941No Hit
GTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA307500.1268571055160687No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259070.10687762707657864No Hit
TATTAGTACTGGTCAGCTTCACGAGTCTTCAGTCCTCGCTTCCACATCCA258940.1068239964303442No Hit
TCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245370.10122578205033426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA564450.059.904421
GTATCAA1486500.043.682151
TATCAAC2359300.028.2319222
ATCAACG2455500.027.4811863
TCAACGC2517450.026.7668344
AACGCAG2554600.026.5632386
CAACGCA2567350.026.3451675
ACGCAGA2681200.025.3208397
CGCAGAG2699700.025.0737938
CAGAGTA2741150.024.85915410
AGAGTAC2737700.024.85708811
GCAGAGT2737500.024.7645919
TACGGGG648650.023.27006115
GTGGTAT90600.023.2267461
TAGTACT105950.022.9903284
GAGTACG2132550.022.33514412
ACGGGAA1250400.022.2304516
TGGTATC90000.022.2075822
CGGGAAA1242300.022.05385817
ACGGGGG439350.021.60732816
Produced by FastQC (version 0.11.4)