FastQCFastQC Report
Mon 22 Feb 2021
ZT4_II_PdfG_plus_S3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZT4_II_PdfG_plus_S3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74643528
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1059150.14189441849533158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA561900.047.172451
GTATCAA1442200.041.4875071
TATCAAC2054500.028.744932
CAACGCA2204500.026.742385
AGAGTAC2217950.026.61572811
AACGCAG2231350.026.5372056
ATCAACG2262650.026.1803363
CAGAGTA2288650.025.84309410
ACGCAGA2289350.025.8377727
GCAGAGT2325400.025.4065119
CGCAGAG2345400.025.1715168
TCAACGC2345900.025.080824
TACGGGG1108900.023.08097815
GTACGGG1710450.022.19987914
AGTACGG1804850.021.19196113
ACGGGGG553500.020.8439916
GAGTACG1913850.020.0671512
GAGTACT1101050.018.84237712
ACGGGGA544200.018.78816216
TACGGGA615300.018.38632215
Produced by FastQC (version 0.11.4)