FastQCFastQC Report
Mon 22 Feb 2021
ZT4_II_PdfG_plus_S3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZT4_II_PdfG_plus_S3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74643528
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1189600.15937081644908316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA527200.046.410361
GTATCAA1306550.040.5855181
TATCAAC1906050.027.5385482
CAACGCA2075400.025.4814415
AACGCAG2105050.025.1922936
AGAGTAC2109750.025.048811
ATCAACG2134050.024.7206673
ACGCAGA2177100.024.2928647
TCAACGC2162000.024.290924
CAGAGTA2188250.024.18367210
GCAGAGT2219250.023.8094589
CGCAGAG2246050.023.5253778
TACGGGG1053900.022.23271415
GTACGGG1575800.020.90817514
ACGGGGG527650.020.08416616
AGTACGG1684200.019.65805213
GAGTACG1794600.018.52081312
ACGGGGA540350.017.86335416
GAGTACT1136200.017.37879212
TACGGGA540200.015.77559215
Produced by FastQC (version 0.11.4)