FastQCFastQC Report
Mon 22 Feb 2021
ZT20_II_PdfG_plus_S11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZT20_II_PdfG_plus_S11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46562038
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTACACCGGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACA1266310.271961893076931No Hit
GATTTATAGGGTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAA1144840.2458741174516459No Hit
ACTTTAGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAA1134230.24359543712412246No Hit
AGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATT968810.20806864166899225No Hit
GATATAACTTTGGTCTGATATTTGTTTTTTCTCATTTTGTCATTATCATT874330.18777743362522062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT804540.17278882853022884No Hit
GTATTTTGACTGTGCAAAGGTAGCATAATCATTAGTCTTTTAATTGAAGG767130.1647543863952003No Hit
GTCAAAATACTGCGGCCATTTAAAATTTTCAGTGGGCAGGTTAGACTTTA732460.15730840647481967No Hit
GTTAATATTTCGTCCAACCATTCATTCCAGCCTTCAATTAAAAGACTAAT710990.15269735401186693No Hit
CATTAATAATGATTAAAAAATTAAGTTACTTTAGGGATAACAGCGTAATT682310.14653782980890998No Hit
AGTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGTATTTTGACTGT652360.14010555122179147No Hit
ATATATAAAGTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGTATT584170.12546057369739702No Hit
GTTCATATCGATAAAAAAGATTGCGACCTCGATGTTGGATTAAGATATAA579270.12440821426244274No Hit
GTATATAATAATGTTAAAATTAATTTATATGAAAAGTTTAAATAAAGAAT566420.12164845533608301No Hit
CTTTAGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAA554980.1191915182063122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA347900.043.3176541
GTATCAA866550.039.9056661
TAGGGTC330000.031.679247
ATAGGGT330800.031.6763156
TATAGGG334200.031.531375
TTATAGG356000.029.894964
TATCAAC1158650.028.9194072
TCAACGC1220550.027.4000994
CAACGCA1239950.027.011355
TGACTAA351300.026.9469144
AACGCAG1260900.026.604796
ATCAACG1264750.026.5786063
CAGAGTA1273350.026.2836210
AGAGTAC1270750.026.2803211
GACTAAA369700.025.76663245
GCAGAGT1311400.025.4906929
ACGCAGA1317500.025.4329247
CGCAGAG1334550.025.1005088
GATTTAT451550.025.0773261
GGGTCTT417700.025.0203449
Produced by FastQC (version 0.11.4)