FastQCFastQC Report
Mon 22 Feb 2021
ZT20_II_PdfG_plus_S11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZT20_II_PdfG_plus_S11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46562038
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTACACCGGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACA1166680.2505646337902993No Hit
ACTTTAGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAA1165490.2503090607846675No Hit
AGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATT1003710.21556401805264622No Hit
GATTTATAGGGTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAA933280.20043796192941557No Hit
GATATAACTTTGGTCTGATATTTGTTTTTTCTCATTTTGTCATTATCATT914490.19640248564721327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT815300.17509972394249582No Hit
GTCAAAATACTGCGGCCATTTAAAATTTTCAGTGGGCAGGTTAGACTTTA742270.1594152730170445No Hit
GTATTTTGACTGTGCAAAGGTAGCATAATCATTAGTCTTTTAATTGAAGG738800.15867003072331157No Hit
GTTAATATTTCGTCCAACCATTCATTCCAGCCTTCAATTAAAAGACTAAT713930.15332876967283948No Hit
CATTAATAATGATTAAAAAATTAAGTTACTTTAGGGATAACAGCGTAATT702590.15089330926623098No Hit
AGTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGTATTTTGACTGT684550.14701890840774626No Hit
GTTCATATCGATAAAAAAGATTGCGACCTCGATGTTGGATTAAGATATAA560880.12045864487288979No Hit
ATATATAAAGTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGTATT558260.11989595472603669No Hit
CTTTAGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAA558030.11984655826276333No Hit
GTATATAATAATGTTAAAATTAATTTATATGAAAAGTTTAAATAAAGAAT549050.11791794852278588No Hit
GATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATGTAAGAA483850.10391512502094517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473410.10167295512279767No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA338450.042.2071341
GTATCAA798700.039.3030051
ATAGGGT285450.029.2551886
TAGGGTC291900.028.7986777
TATAGGG293850.028.704855
TATCAAC1082050.028.222142
TTATAGG316100.026.7286874
TCAACGC1158050.026.4330334
CAACGCA1182350.026.1294965
AACGCAG1200950.025.8179826
ATCAACG1202100.025.6450423
TGACTAA292950.025.5303544
CAGAGTA1212250.025.40547610
AGAGTAC1213500.025.36518711
ACGCAGA1258900.024.5500437
GCAGAGT1255250.024.5351039
TATAACT313850.024.411273
CGCAGAG1277750.024.122218
GACTAAA313550.023.92022545
ATATAAC340950.023.0563682
Produced by FastQC (version 0.11.4)