FastQCFastQC Report
Mon 22 Feb 2021
ZT16_II_PdfG_plus_S9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZT16_II_PdfG_plus_S9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81519874
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTACACCGGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACA5550530.6808805911549863No Hit
GATTTATAGGGTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAA4806400.589598556052724No Hit
ACTTTAGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAA4658680.5714778214696455No Hit
AGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATT4076560.5000694677226808No Hit
GTATTTTGACTGTGCAAAGGTAGCATAATCATTAGTCTTTTAATTGAAGG3100300.3803121678033997No Hit
GTCAAAATACTGCGGCCATTTAAAATTTTCAGTGGGCAGGTTAGACTTTA3061450.3755464587690604No Hit
CATTAATAATGATTAAAAAATTAAGTTACTTTAGGGATAACAGCGTAATT3045640.3736070543975571No Hit
GTTAATATTTCGTCCAACCATTCATTCCAGCCTTCAATTAAAAGACTAAT2912940.3573288153021434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860850.35093896244245915No Hit
AGTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGTATTTTGACTGT2732160.335152627934631No Hit
GTATATAATAATGTTAAAATTAATTTATATGAAAAGTTTAAATAAAGAAT2536320.31112903830052535No Hit
ATATATAAAGTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGTATT2424760.2974440318688422No Hit
GTTCATATCGATAAAAAAGATTGCGACCTCGATGTTGGATTAAGATATAA2349730.2882401412936433No Hit
CTTTAGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAA2278430.2794938078535303No Hit
GATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATGTAAGAA1910520.23436248196360068No Hit
GATATAACTTTGGTCTGATATTTGTTTTTTCTCATTTTGTCATTATCATT1782190.2186203084661294No Hit
ATATAAAGTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGTATTTT1768270.21691274939899932No Hit
AAATAATATTCGCCTGTTTAACAAAAACATGTCTTTTTGAATTATATATA1745790.21415513964116287No Hit
ATATAATTATTTTGATATTTGGTCCTTTCGTACTAAAATATCATAATTTT1720610.21106632230565026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1682520.20639384207095315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1522510.18676549966208242No Hit
GGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTGC1448110.17763889085500795No Hit
ATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAA1440830.17674585709001464No Hit
GTTTATTAGTATTGTAAAAGAAAATTGAAATAATTTGAAAAAATTTTATT1381930.16952062511774738No Hit
CAGTTAATATTTCGTCCAACCATTCATTCCAGCCTTCAATTAAAAGACTA1381870.16951326494935456No Hit
GACTTAAAATTTGAACGGCTACACCCAAAATTATATCTTAATCCAACATC1343670.16482729107260397No Hit
ATTTTGATATTTGGTCCTTTCGTACTAAAATATCATAATTTTTTAAAGAT1323920.16240456897664No Hit
ATAATATTCTTATAAAAAATATAATTATTTTGATATTTGGTCCTTTCGTA1310610.16077183828817007No Hit
TTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGACCTCGATGTTGGAT1250990.15345828429518918No Hit
AGTTAATATTTCGTCCAACCATTCATTCCAGCCTTCAATTAAAAGACTAA1211210.14857849265076145No Hit
AAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATG1193160.1463643086592602No Hit
ATAAAAAAGATTGCGACCTCGATGTTGGATTAAGATATAATTTTGGGTGT1192050.14622814554399335No Hit
GTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGTATTTTGACTGTG1177700.14446783860338155No Hit
TTTTAAGTCTGTTCGACTTTTAAATTCTTACATGATCTGAGTTCAAACCG1155270.14171636231920573No Hit
GGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAAT1142100.1401008053569857No Hit
CTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGG1121640.13759098793504024No Hit
ATGTTGGATTAAGATATAATTTTGGGTGTAGCCGTTCAAATTTTAAGTCT1120950.13750634599852302No Hit
ATATAATTTTGGGTGTAGCCGTTCAAATTTTAAGTCTGTTCGACTTTTAA1118570.1372143926522752No Hit
ATATAAAGATTTATAGGGTCTTCTCGTCTTTTAAATAAATTTTAGCTTTT1106360.13571659838434No Hit
TATATAAAGTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGTATTT1092910.13406669396962023No Hit
GTACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTGGCTTACAC1074560.13181570913615495No Hit
TATTTGGTCCTTTCGTACTAAAATATCATAATTTTTTAAAGATAGAAACC1073980.1317445608416912No Hit
TCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCC1067810.1309876901919647No Hit
GTGCAAAGGTAGCATAATCATTAGTCTTTTAATTGAAGGCTGGAATGAAT1055530.12948130906090458No Hit
ATATTCGCCTGTTTAACAAAAACATGTCTTTTTGAATTATATATAAAGTC1046890.1284214448123411No Hit
GGATTAAGATATAATTTTGGGTGTAGCCGTTCAAATTTTAAGTCTGTTCG1037350.12725117803788558No Hit
GTTTAAATAAAGAATTCGGCAAAAATAATATTCGCCTGTTTAACAAAAAC1010710.1239832632714815No Hit
ATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAA1007750.12362016163076994No Hit
CCTTTGCACAGTCAAAATACTGCGGCCATTTAAAATTTTCAGTGGGCAGG1004020.12316260449568407No Hit
TTTATAGGGTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAA1002000.12291481215979308No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA971840.11921510084767796No Hit
ACCTTTGCACAGTCAAAATACTGCGGCCATTTAAAATTTTCAGTGGGCAG965170.11839689546134481No Hit
ATTTTATTGGGGTGATATTAAAATTTAAAAAACTTTTAATTTTTTAAAAA953200.11692854186698079No Hit
TTATTAATGGATCAAATATTCATAAATTTATGTTTTTAAAAAATTAAAAG943300.11571411408216849No Hit
TGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCCATTTAAAATTTT925730.11355881143780963No Hit
TATTTGATCCATTAATAATGATTAAAAAATTAAGTTACTTTAGGGATAAC924780.11344227543825694No Hit
GGTTAGACTTTATATATAATTCAAAAAGACATGTTTTTGTTAAACAGGCG923960.11334168647022197No Hit
ATGTAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCC911740.11184266550755464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA906290.11117411687854178No Hit
TACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTGGCTTACACC899460.1103362843764945No Hit
AGATATAATTTTGGGTGTAGCCGTTCAAATTTTAAGTCTGTTCGACTTTT885310.10860051133052535No Hit
AAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATGTAAGAATTTAA849280.10418073021064778No Hit
CCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGA845100.1036679718126159No Hit
GACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCCATTTAA826770.10141944036861489No Hit
ATATAATAATGTTAAAATTAATTTATATGAAAAGTTTAAATAAAGAATTC825740.10129309081120513No Hit
ATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCAC825630.10127959716915166No Hit
CTCTCGAATTGTGTCCGTTGGTAGCCGAAGTTACGATTGCAAAACTTTCG821790.10080854639201234No Hit
ATTATATATAAAGTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGT819920.10057915447710335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1103950.051.177131
GTATCAA2756450.041.4066961
ATAGGGT1284700.034.025876
TAGGGTC1284800.033.9446877
TATAGGG1300550.033.6320425
TTATAGG1338400.032.6358344
GATTTAT1406300.031.9277321
AGGGTCT1368600.031.9025678
GGGTCTT1383300.031.4099779
TTGACTA1296700.030.47911643
TGACTAA1323200.029.97637444
GACTAAA1357400.029.4687845
CGTCTTT1389400.029.3157818
GGTCTTC1489450.029.03613910
CTCGTCT1403750.028.78036916
TATCAAC3921250.028.5598182
TCGTCTT1419150.028.48012717
GTATATA837700.028.431811
GTCTTCT1524850.028.13337711
TCAACGC3974650.028.0863844
Produced by FastQC (version 0.11.4)