FastQCFastQC Report
Mon 22 Feb 2021
ZT12_II_PdfG_plus_S7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZT12_II_PdfG_plus_S7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25850541
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4428511.713120820179353No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3276121.2673313103969468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3072941.1887333421764752No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2559770.9902191215263154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2171760.8401216825597576No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2138260.8271625727291355No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1705830.6598817409662722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1639830.6343503604044496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1197880.46338682041509305No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1062330.4109507804885012No Hit
TATTAGTACTGGTCAGCTTCACGAGTCTTCAGTCCTCGCTTCCACATCCA778250.30105752912482564No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT764220.2956301765599413No Hit
GAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA722810.2796111694528946No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA637670.24667568852814337No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532290.20591058423109987No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420800.1627818930365906No Hit
GTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA418120.16174516425014085No Hit
GTATCAACGCAGAGTACGGGGGGCCCCTCCTTGTCATGTCTGCCGGCATG361710.13992357065177088No Hit
GTATCAACGCAGAGTACGGGGGGTGTGAGGCCCCCAGCTAGAACACTGGG358520.13868955392461613No Hit
CATATAGACGAACCAGTGTGGATGCTGGACCGTAGAGGGTGAGAGTCCCG331300.12815979363836139No Hit
GTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGGGATTCCTCGG312650.12094524443414936No Hit
GTACTGATCCGCTATCGGTCAACAGGTAGTATTCAGGCTTACCAGGTGGT300320.11617551833828159No Hit
TCCTTGTCATGTCTGCCGGCATGGCGCAGTGAGGTGCGGGCATATAGACG285920.11060503530661119No Hit
GCCTATTAGTACTGGTCAGCTTCACGAGTCTTCAGTCCTCGCTTCCACAT281410.10886039096821996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA912300.062.5710871
GTATCAA2467750.043.7989581
TGGTATC104150.036.2302362
GTGGTAT114600.032.9996721
CCTATTA75950.029.6343152
TAGTACT234050.028.9941444
TATTAGT227000.028.830621
GCCTATT80050.027.9955581
ATCAACG3960750.027.8584163
TATCAAC3982450.027.557542
ATTAGTA241950.027.5167122
TCAACGC4025900.027.3530564
CAACGCA4098900.026.9498425
AACGCAG4144950.026.7632546
AGTACTG269450.025.5748375
ACGCAGA4411600.025.1555127
CGCAGAG4454650.024.8607448
AGAGTAC4551900.024.41833111
CAGAGTA4556600.024.4101510
GCAGAGT4543800.024.36959
Produced by FastQC (version 0.11.4)