FastQCFastQC Report
Mon 22 Feb 2021
ZT12_II_PdfG_plus_S7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZT12_II_PdfG_plus_S7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25850541
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4101031.5864387519007823No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3794201.4677449110252663No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3750941.4510102515842898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2928491.1328544342650313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360750.9132304039594373No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1847620.7147316568732546No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1821910.7047860236271264No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1749070.6766086636252603No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1417620.5483908441219857No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1347340.5212037922146388No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260480.4876029480388825No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT887210.34320751739779837No Hit
TATTAGTACTGGTCAGCTTCACGAGTCTTCAGTCCTCGCTTCCACATCCA860050.3327009674575089No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT628900.24328310962621635No Hit
GAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA487210.1884718776291761No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA386370.14946302284350646No Hit
CATATAGACGAACCAGTGTGGATGCTGGACCGTAGAGGGTGAGAGTCCCG371160.1435792001413046No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344430.1332389910137664No Hit
GTATCAACGCAGAGTACGGGGGGTGTGAGGCCCCCAGCTAGAACACTGGG318520.12321598994775389No Hit
GTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGGGATTCCTCGG315950.1222218134622405No Hit
GTACTGATCCGCTATCGGTCAACAGGTAGTATTCAGGCTTACCAGGTGGT314510.12166476515907347No Hit
GCCTATTAGTACTGGTCAGCTTCACGAGTCTTCAGTCCTCGCTTCCACAT304760.11789308393971329No Hit
GTATCAACGCAGAGTACGGGGGGCCCCTCCTTGTCATGTCTGCCGGCATG296470.1146861878055086No Hit
GTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA295820.1144347423908846No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294440.11390090443368284No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289720.1120750238844131No Hit
TCCTTGTCATGTCTGCCGGCATGGCGCAGTGAGGTGCGGGCATATAGACG285350.11038453701994089No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279680.10819115932622068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA869850.063.034591
GTATCAA2264600.042.7018931
TGGTATC94500.031.6667632
CCTATTA81750.031.596432
GCCTATT84150.030.6573071
TAGTACT254250.030.4636754
TATTAGT250600.030.0874421
ATTAGTA268150.028.7806242
GTGGTAT105200.028.6156331
ATCAACG3633100.027.142463
AGTACTG300150.027.1108445
TATCAAC3683900.026.658382
TCAACGC3718450.026.4892854
CAACGCA3817100.026.0199625
AACGCAG3870500.025.6625866
TACTGGT307600.025.5198867
GAGTCTT305600.024.72717923
ACTGGTC321750.024.6478378
GTACTGG326650.024.3326596
CACGAGT311600.024.31773420
Produced by FastQC (version 0.11.4)