FastQCFastQC Report
Mon 22 Feb 2021
ZT0_II_PdfG_plus_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZT0_II_PdfG_plus_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18751397
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3156531.683357245329508No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2172951.1588203268268493No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1672060.8916988958209353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1555210.8293835387304743No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1487550.7933008937947397No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1181950.6303263698166062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1151870.6142848983465071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT883250.4710315716743664No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT657220.35049121940087985No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT557930.2975404979159686No Hit
TATTAGTACTGGTCAGCTTCACGAGTCTTCAGTCCTCGCTTCCACATCCA454540.24240327267349734No Hit
GAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412110.21977562525074798No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408960.21809575041262258No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA395210.2107629634208054No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279530.14907155984164808No Hit
GTATCAACGCAGAGTACGGGGGGTGTGAGGCCCCCAGCTAGAACACTGGG244450.13036362037452462No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222650.11873781990749809No Hit
GTATCAACGCAGAGTACGGGGGGCCCCTCCTTGTCATGTCTGCCGGCATG210180.11208764872291915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA579250.060.99951
GTATCAA1472050.042.183471
TGGTATC78400.032.815222
GTGGTAT83500.031.2412151
TAGTACT136750.028.5860044
ATTAGTA136050.028.1978532
TATTAGT136550.027.5147861
ATCAACG2392700.026.6428853
TATCAAC2384450.026.4982432
CCTATTA48900.026.1269112
TCAACGC2442450.026.0449874
AACGCAG2499550.025.659016
CAACGCA2491450.025.6387885
ACGCAGA2645250.024.256567
CGCAGAG2665700.024.0077088
AGTACTG164900.023.982645
GCCTATT54200.023.9034481
CAGAGTA2710250.023.73753210
AGAGTAC2708150.023.72703611
GCAGAGT2706500.023.6423559
Produced by FastQC (version 0.11.4)