FastQCFastQC Report
Mon 22 Feb 2021
ZT0_II_PdfG_plus_S1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZT0_II_PdfG_plus_S1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18751397
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2723681.4525211108271028No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2261351.2059634810142412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2223151.1855916655169745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1686910.8996183057720979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370970.7311295259761179No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1115190.5947236891203359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1114140.5941637308409609No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1063950.5673977250868295No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960640.5123031633323106No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA831370.44336429973724095No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT738910.3940559735362651No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT497570.26535089625588965No Hit
TATTAGTACTGGTCAGCTTCACGAGTCTTCAGTCCTCGCTTCCACATCCA487000.25971398291018No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352830.18816198067802628No Hit
GAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA253610.1352485897450734No Hit
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227530.12134029267259394No Hit
GTATCAACGCAGAGTACGGGGGGTGTGAGGCCCCCAGCTAGAACACTGGG219650.11713793910928343No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206800.11028511635693063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA548100.061.3831831
GTATCAA1339600.040.972651
TATTAGT143400.028.8267861
TAGTACT149250.028.7264064
ATTAGTA147450.028.6028882
TGGTATC68550.027.9284922
GTGGTAT73550.026.1267321
ATCAACG2161700.026.0119573
CCTATTA47750.025.8743212
TATCAAC2168450.025.7732262
TCAACGC2216600.025.3392124
AGTACTG184200.024.909875
CAACGCA2279250.024.820775
AACGCAG2296950.024.6416286
GAGTCTT179550.023.13912223
CACGAGT179350.023.02782420
ACGAGTC182050.022.85938821
ACGCAGA2501100.022.628957
TTAGTAC196650.022.6032283
TCACGAG185050.022.43187319
Produced by FastQC (version 0.11.4)