FastQCFastQC Report
Thu 22 Sep 2022
10_S28_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename10_S28_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85447305
Sequences flagged as poor quality0
Sequence length101
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC7452293287.21507600503024TruSeq Adapter, Index 3 (100% over 50bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC5179000.6061045459537899TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC3197280.37418149115410954TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGACATCTCGTATGC2623350.3070137788429957TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCATCTCGTATGC2447030.286378838981522TruSeq Adapter, Index 3 (98% over 50bp)
GATCGAAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2432240.2846479476444576TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGAATCTCGTATGC2431190.28452506489233337TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGGCATCTCGTATGC2355830.2757055942255873TruSeq Adapter, Index 3 (98% over 50bp)
GNTCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2252590.26362329391196127TruSeq Adapter, Index 3 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2128090.24905291044580047TruSeq Adapter, Index 3 (100% over 49bp)
GATCAGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2097540.24547760751494735TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1800730.210741579269235TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGTATGC1754650.20534878191886802TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAACACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1700170.19897292255150706TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGG1659120.19416879209941143TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACATCTGAACTCCAGTCACTTAGGCATCTCGTATGC1643430.1923325726890977TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATGCATCTCGTATGC1604370.18776133431007566TruSeq Adapter, Index 3 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1578410.18472320455279426TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAAGCATCTCGTATGC1255400.14692095906360067TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC1217790.14251941591370262TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGCATCTCGTATGC1164980.13633899863781543TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGCCATCTCGTATGC1063370.12444745916796324TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGTCATCTCGTATGC1027220.12021678155911411TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTAAACTCCAGTCACTTAGGCATCTCGTATGC1025290.1199909113575905TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAATCACTTAGGCATCTCGTATGC1016350.11894465249664692TruSeq Adapter, Index 3 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC946610.11078289713174687TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATGC921550.10785009544771482TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)