FastQCFastQC Report
Thu 6 Apr 2023
SRR1971631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189593674
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG272150.019.4685363
GTATCGA323050.017.2161924
TAAGGCG644250.015.7407268
CCCGTAT373150.015.5220121
CTTGGAT1411150.013.5960931
TTAAGGC903450.012.2709247
CGACCGT100450.012.0750931
GGATTAA997300.012.0208854
CCGTATC469900.011.98574352
GACGCTA195850.011.8103734
TACCGTA279900.011.707369
TTGGATT1401250.011.65877152
AAGGCGA903500.011.5154889
CCGGTAA169550.011.1467681
ATCGAAG640800.010.8799346
GATTAAG1333600.010.6037635
ACGCTAC226600.010.4770565
CTACCGT333800.010.0986188
GTTCGAT212750.09.9882541
CGGTAAT183600.09.62799551
Produced by FastQC (version 0.11.4)