FastQCFastQC Report
Thu 6 Apr 2023
SRR1971631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189593674
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG276650.018.636073
GTATCGA328450.016.5701244
CCCGTAT370050.015.5374521
CCGTATC389050.014.1096352
TAAGGCG654250.013.7062788
TACCGTA289700.012.8649939
CGACCGT100150.012.3927231
CTTGGAT1478000.012.1666441
GACGCTA193850.011.5648264
CTACCGT340650.011.3685148
TTAAGGC898000.011.1006717
CCGGTAA177700.010.8734691
GGATTAA972400.010.8265374
TTGGATT1442150.010.6271972
CGCGTAA27550.010.4093111
ATCGAAG651200.010.2051626
ACGCTAC225500.010.1917625
AAGGCGA914800.010.1210379
GTTCGAT217850.09.9700691
GATTAAG1306000.09.6984555
Produced by FastQC (version 0.11.4)