FastQCFastQC Report
Thu 6 Apr 2023
SRR1971630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157762296
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG206450.016.9451273
GTATCGA249450.014.6461924
CCCGTAT282350.014.2718251
TAAGGCG536750.013.664598
CTTGGAT1110000.012.7632911
TACCGTA242250.012.2264259
TTGGATT1063500.011.3052462
GGATTAA747100.011.1147364
TTAAGGC717550.011.0669917
GTTCGAT196500.010.7680361
CTACCGT283600.010.6597948
GATTAAG1057400.09.7473645
AAGGCGA796000.09.6862019
CCGGTAA158150.09.6627481
CGACCGT98050.09.4951991
CCGTATC395400.09.4540362
ATCGTTC245450.09.3090766
CGGTAAT163750.09.3035821
CTCGGAT416900.09.248441
CTCAGAT1337750.09.0613821
Produced by FastQC (version 0.11.4)