FastQCFastQC Report
Thu 6 Apr 2023
SRR1971630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157762296
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG209000.016.1689473
GTATCGA244350.014.3491324
CCCGTAT275050.013.4839921
TACCGTA262600.013.1369179
TAAGGCG509050.012.2795758
CCGTATC295900.012.0716822
CTTGGAT1143150.011.7437851
CTACCGT299850.011.6930268
CGGTAAT159450.010.5538661
GTTCGAT188700.010.5371111
ACTACCG306600.010.2092637
TTGGATT1067900.010.1975212
TTAAGGC690300.010.1923947
GGATTAA727100.010.1421024
CGACCGT99050.09.49136451
CCGTATG187400.09.354732510-11
GATTAAG986450.09.3242515
TCAATCG299350.09.1849283
CAATCGG306050.09.1834944
CTCGGAT419550.09.153591
Produced by FastQC (version 0.11.4)