FastQCFastQC Report
Thu 6 Apr 2023
SRR1971629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189993197
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG370700.021.1182523
GTATCGA413350.019.405614
TAAGGCG845850.018.087228
CGACCGT134550.017.5424631
GGATTAA934800.017.3526824
TTAAGGC926600.017.1349687
GACGCTA336600.017.0276934
TTGGATT1235150.016.8561652
TACCGTA288750.016.8145129
CTTGGAT1440250.016.6397691
CCCGTAT509700.016.4385551
CCGTATC534100.015.1065622
ACGCTAC378700.014.9829975
CTACCGT333550.014.7254838
GTGGAAT781250.014.1793341
AAGGCGA1134450.013.7466529
CCGGTAA183100.012.9423381
TGGATTA1260700.012.79233
GGAATTC818700.012.78399853
ATCGAAG711500.012.5114816
Produced by FastQC (version 0.11.4)