FastQCFastQC Report
Thu 6 Apr 2023
SRR1971629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189993197
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG362350.019.9114763
TACCGTA312400.019.5123659
GTATCGA402950.018.3835874
CTACCGT362000.017.0725468
TAAGGCG824050.016.9730728
CGACCGT135300.016.8834721
CCGTATC451100.016.4004332
GGATTAA904300.016.3728124
TTAAGGC902550.016.0748337
TTGGATT1293500.014.97475052
CTTGGAT1500650.014.9372681
CCCGTAT509750.014.8822831
GACGCTA328800.014.8814134
GTGGAAT798500.014.2097231
CCGGTAA186800.013.7133291
CGTATGG217950.013.53106112-13
CCGTATG245300.013.42106710-11
ACGCTAC370800.013.3980445
ACTACCG471400.013.3796287
GGAATTC816500.013.3208073
Produced by FastQC (version 0.11.4)