FastQCFastQC Report
Thu 6 Apr 2023
SRR1971628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202795863
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT137950.027.7118991
GACCGTT167850.019.4688452
ACCGTTG172150.018.9271243
CATACGA158500.018.6653714-15
GACGCTA402450.018.166034
CTACCGT420200.018.0762168
TACCGTA428700.017.8162369
CCGGTAA187050.017.5718271
ACGCTAC426450.017.0865545
CGGCATA189600.016.06217812-13
ATACGAG182500.016.00636716-17
GCATACG188200.015.99446314-15
CTTGGAT1342100.015.8046681
TTAAGGC989350.015.0986877
CCGTATG275550.014.89072110-11
CGGTAAT215400.014.4070472
ACTACCG525600.014.3534067
GGATTAA1089250.014.3215644
TTGGATT1368600.014.1786442
CGCTACT524000.014.09213356
Produced by FastQC (version 0.11.4)