FastQCFastQC Report
Thu 6 Apr 2023
SRR1971628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202795863
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT128550.027.059591
CTCGTAT498600.019.49331742-43
TACCGTA474900.018.9126459
CTACCGT475850.018.8057488
GACGCTA379950.018.3836364
TATGCCG550800.018.2346146-47
ACCGTTG166350.017.8589383
CCGGTAA186050.017.6860121
CGTATGC574700.017.43949544-45
GACCGTT168700.017.2200622
ACGCTAC412450.017.0593835
CCGTATG312550.015.7290410-11
ACTACCG577950.015.6787187
TCACGAT661850.015.38117930-31
CGGTAAT210550.014.7796492
CTTGGAT1363400.014.6943941
TTAAGGC954850.014.5943027
CGTATGG300900.014.16677512-13
TCGTATG495250.014.15422942-43
CGCTACT502200.014.1316876
Produced by FastQC (version 0.11.4)