FastQCFastQC Report
Thu 6 Apr 2023
SRR1971627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170806713
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG275650.020.2437933
GTATCGA319000.018.0535244
CCCGTAT350050.017.0565661
CATACGA183550.016.88473914-15
CGACCGT114300.016.7404121
AGACGGC511400.015.8551659
AAGACGG521100.015.5242358
GCATACG220650.015.47268214-15
ATACGAG197850.014.97750216-17
GACGCTA199400.013.674754
CGGCATA256700.013.14617512-13
TACCGTA236450.012.5041369
ACGCTAC227000.012.2588385
TGGTCGC425350.012.09756242-43
CTTGGAT1145050.011.97650051
GTTCGAT233750.011.8864961
TAAGGCG510400.011.6409078
CTACCGT255900.011.4804828
ATCGAAG596950.011.4798546
CGCCGTA510750.011.102262546-47
Produced by FastQC (version 0.11.4)