FastQCFastQC Report
Thu 6 Apr 2023
SRR1971627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170806713
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1771180.10369498767885078TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT581450.022.05006842-43
TATGCCG615900.021.62479246-47
CGTATGC613400.021.56381844-45
CGTATCG273900.018.9102063
TCGTATG578900.017.15027642-43
GTATCGA320900.016.800574
GATCGGA1169950.016.7527081
ATCGGAA1203150.016.278352
CGACCGT112800.016.2090231
CCCGTAT353750.015.9972881
TCGGAAG1281500.015.4041383
CCGTATC363900.014.8791152
CACGTCT910000.014.87909914-15
CCGTCTT919050.014.73726850-51
CACACGT922600.014.68843612-13
ATCTCGT895700.014.51353140-41
TCTCGTA675500.014.39175140-41
TACCGTA256850.014.2542289
GACGCTA189550.013.6633794
CGGAAGA1506450.013.3760874
Produced by FastQC (version 0.11.4)