FastQCFastQC Report
Thu 6 Apr 2023
SRR1971626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84236891
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA134550.022.56389614-15
AAGACGG285800.022.4235068
ATACGAG136650.021.41303316-17
AGACGGC298600.021.3676159
GCATACG155500.020.0981714-15
CGGCATA167850.018.43156212-13
CGTATCG103100.016.963613
TACGAGA175600.016.47600716-17
CCCGTAT143250.014.9008131
GAAGACG459450.014.0817387
GTATCGA133750.013.8496074
TACCGTA113600.013.451519
CGCCGTA335850.013.23844546-47
CGAGATT223000.013.1539918-19
TAAGGCG213500.012.9934368
CCGTATC358300.012.50123448-49
TGGTCGC308750.012.48759942-43
GTCGCCG351250.012.26918844-45
CGACCGT49850.012.1667131
CTTGGAT583750.012.0249041
Produced by FastQC (version 0.11.4)