FastQCFastQC Report
Thu 6 Apr 2023
SRR1971626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1971626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84236891
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG102750.019.2571493
CTCGTAT359900.016.7741442-43
CCCGTAT143100.016.0965181
GTATCGA132100.015.1919564
TACCGTA124700.014.73662959
TATGCCG426100.014.64235346-47
CGTATGC434900.014.29203744-45
CCGTATC152350.013.8514942
CTACCGT140500.013.4139358
TCGTATG365850.012.40329942-43
ATCTCGT504200.012.14123840-41
TAAGGCG209250.011.63462458
ATCGGAA747450.011.6013092
GTGGAAT441750.011.5141
CACGTCT546800.011.501614-15
TCGGAAG767250.011.491793
GATCGGA760650.011.3959771
CCGTCTT564550.011.28873250-51
TCTCGTA394750.011.26307940-41
GACGCTA90950.011.2136154
Produced by FastQC (version 0.11.4)