FastQCFastQC Report
Mon 7 Oct 2024
quad_212_S17_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_212_S17_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59517609
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40768966.8498988257408No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC2661860.4472390683570639No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC2008970.3375421213577313No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC1609410.27040904818605865No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC1577290.2650123260159863No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG1231440.20690347288648642No Hit
CCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCC982970.16515616411942893No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT931920.1565788706330592No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC916970.15406700897544456No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT766710.12882069909764016No Hit
CTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCT726330.12203615235954791No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC684150.11494917411752881No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA682440.11466186418879831No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT622170.10453544933231441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCA379800.034.5769654
GCGCACG386600.034.0301936
CGGTGGC427500.033.0501861
GGCGCAC454000.029.009475
CGCGGTG91350.028.315171
GGTGGCG504350.027.8731272
GTGGCGC532750.026.253633
CGCACGC540650.024.535717
GCCCTCT887900.023.182383
GCACGCC587950.022.6952618
CGGAACG80500.022.34471
CACGCCT606550.021.959999
TCCCACG1351350.019.959172
GGCGATC53900.019.0194382
CGAGGCG144750.018.2300321
CCCACGT1477900.018.1601833
CTCCCAC1564050.018.039831
ACGTCCG1517100.017.7754276
CCTTATA222300.017.5068246
CCACGTC1535950.017.4954624
Produced by FastQC (version 0.11.4)