FastQCFastQC Report
Mon 7 Oct 2024
quad_211_S16_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_211_S16_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61695375
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36349855.89182738576433No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC1708340.276899200304723No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC1187250.19243743959737664No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG1074220.17411677941823028No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC1041330.16878574771609703No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC1018960.1651598681424661No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT903090.14637888172330582No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC794110.12871467269629208No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA787430.12763193351203392No Hit
CAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAAC744660.12069948517210569No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT716070.11606542629816255No Hit
CCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCC631920.10242583013718613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCA341800.032.1310354
GCGCACG356900.030.9178876
CGGTGGC382250.030.9160881
CGCGGTG88000.029.5549761
CGCACGC492900.022.5122627
CCTTATA261100.020.6856336
GGCCTTA268350.020.3036294
CGATACG55500.020.1814526
GCACGCC554400.020.1530578
CACGCCT555050.020.0265059
CAACCCG237750.019.7226859
CGGAACG149400.019.31461
GCCTTAT285650.019.2068885
GATTCAA295200.018.2318235
GCCCTCT694500.017.0639553
CGACAAT248600.016.914741
GTTATAC349700.016.8154745
CCCACGT1002750.016.7881153
ACGTCCG1012300.016.7703886
TCCCACG1043200.016.6742
Produced by FastQC (version 0.11.4)