FastQCFastQC Report
Mon 7 Oct 2024
quad_211_S16_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_211_S16_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61695375
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGGGG30793114.991153712899873TruSeq Adapter, Index 14 (97% over 43bp)
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA2455040.39792934235345845No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT2140870.34700656248543754No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT1445100.23423149628314927No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG1430590.23187961820476818No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA1426210.23116967844023967No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1189980.19287993629992523No Hit
CTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTATTTAT1170210.189675482157293No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT972520.1576325615980128No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC937760.15199842775896896No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC870680.1411256516392031No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG850960.13792930183178237No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT798270.12938895338588996No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA794030.12870170575995363No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA777270.1259851325970545No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT741060.12011597303687674No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT691070.11201325869240604No Hit
CTCCTTAGTCCTTTAGTTTCATAAAGGGTATAGTAATGTTCTTTTATAAG657000.10649096467928107No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA637230.1032865105366488No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG622350.10087466037770254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA4418500.074.854059
AGAGCAC4441650.074.5509648
CGGAAGA4678900.070.899174
AAGAGCA4698950.070.586597
ATCGGAA4723150.070.215232
TCGGAAG4784450.069.452423
GATCGGA4788000.069.192051
GAAGAGC5173950.064.106896
TCGCTAT196200.050.3051452
GGAAGAG6645600.050.223285
CTCGCTA200750.049.4693261
CGCTATG212650.046.300753
GTTCCAT3777800.044.22193534-35
ACAGTTC3781350.043.06559832-33
CAGTTCC3925000.042.3560832-33
CACAGTT3949800.042.0847730-31
CGTCTGA3908000.042.0446616-17
ACGTCTG3977700.041.86705414-15
TCCAGTC4031100.041.6686424-25
AGTTCCA3925500.041.33180234-35
Produced by FastQC (version 0.11.4)