FastQCFastQC Report
Mon 7 Oct 2024
quad_210_S21_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamequad_210_S21_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57760219
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10101961.7489476624041194No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC1504050.2603954808412344No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC937950.16238684967589892No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT840120.14544958702459215No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG804620.13930348844418336No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC799500.13841706521230468No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC795080.13765183265665942No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC746680.12927236304280632No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT671230.11620973944021923No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT633450.10966890551436448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACG290650.029.9637136
TGGCGCA304350.028.3498294
CGGTGGC381050.024.5116331
CGCGGTG88950.023.5833471
GGCGCAC380700.022.9509565
GGTGGCG430350.021.4169332
GTGGCGC442750.020.731363
CGCACGC435600.020.1019577
TCCCACG761750.019.7005922
ACGTCCG796400.018.9983946
GCACGCC474600.018.6169328
CCCACGT804300.018.5875593
CACGCCT490850.018.146059
CACGTCC838250.017.9989385
CCACGTC837200.017.8967934
CGTCCGG856400.017.689527
GTCCGGG889800.017.2488638
CTCCCAC1020500.016.375031
GCCCTCT631850.015.4357493
ACGCCTG566950.014.6662544
Produced by FastQC (version 0.11.4)